Detailed information of TA system TA173223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 109492..110402
• Replicon: plasmid unnamed1
• Accession: NZ_CP061949
• Organism: Klebsiella pneumoniae strain IR5065

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: H4I82_RS27040
• Protein ID: WP_004181896.1
• Coordinates: 109492..109962 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: H4I82_RS27045
• Protein ID: WP_004181895.1
• Coordinates: 109959..110402 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I82_RS27015	104987..105364	+	378	WP_004181901.1	hypothetical protein	-
H4I82_RS27020	105562..106536	-	975	WP_004196336.1	hypothetical protein	-
H4I82_RS27025	107139..107297	-	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
H4I82_RS27030	107369..108550	+	1182	WP_004883380.1	IS481 family transposase	-
H4I82_RS27035	109122..109382	+	261	WP_004026352.1	hypothetical protein	-
H4I82_RS27040	109492..109962	-	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
H4I82_RS27045	109959..110402	-	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
H4I82_RS27050	110503..111774	-	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
H4I82_RS27055	111774..112196	-	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
H4I82_RS27060	112551..113120	-	570	Protein_113	transposase	-
H4I82_RS27065	113122..113337	+	216	Protein_114	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / ant(3'')-Ia / qacE / sul1 / aph(3')-Ia	iutA / iutA / iucD / iucC / iucC / iucB / iucA / iucA / rmpA / rmpA2 / iutA	1..295331	295331
	-	inside	IScluster/Tn	-	-	107369..116615	9246

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T173223 WP_004181896.1 NZ_CP061949:c109962-109492 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T173223 NZ_CP080120:c2099905-2099531 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT173223 WP_004181895.1 NZ_CP061949:c110402-109959 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT173223 NZ_CP080120:c2100362-2099994 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9