Detailed information of TA system TA173222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5287390..5288015
• Replicon: chromosome
• Accession: NZ_CP061948
• Organism: Klebsiella pneumoniae strain IR5065

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H4I82_RS26130
• Protein ID: WP_062921072.1
• Coordinates: 5287390..5287773 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: H4I82_RS26135
• Protein ID: WP_004150355.1
• Coordinates: 5287773..5288015 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I82_RS26115	5284756..5285658	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
H4I82_RS26120	5285655..5286290	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
H4I82_RS26125	5286287..5287216	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
H4I82_RS26130	5287390..5287773	-	384	WP_062921072.1	type II toxin-antitoxin system VapC family toxin	Toxin
H4I82_RS26135	5287773..5288015	-	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
H4I82_RS26140	5288220..5289137	+	918	WP_062921073.1	alpha/beta hydrolase	-
H4I82_RS26145	5289151..5290092	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
H4I82_RS26150	5290137..5290574	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H4I82_RS26155	5290571..5291431	-	861	WP_004146232.1	virulence factor BrkB family protein	-
H4I82_RS26160	5291425..5292024	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T173222 WP_062921072.1 NZ_CP061948:c5287773-5287390 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDALILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T173222 NZ_CP080120:2088744-2088998 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT173222 WP_004150355.1 NZ_CP061948:c5288015-5287773 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT173222 NZ_CP080120:2088496-2088747 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9