Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4046555..4047174 Replicon chromosome
Accession NZ_CP061948
Organism Klebsiella pneumoniae strain IR5065

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag H4I82_RS20145 Protein ID WP_002892050.1
Coordinates 4046956..4047174 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag H4I82_RS20140 Protein ID WP_002892066.1
Coordinates 4046555..4046929 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I82_RS20130 4041707..4042900 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H4I82_RS20135 4042923..4046069 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
H4I82_RS20140 4046555..4046929 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
H4I82_RS20145 4046956..4047174 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
H4I82_RS20150 4047333..4047899 + 567 WP_002892030.1 maltose O-acetyltransferase -
H4I82_RS20155 4047871..4048011 - 141 WP_004147370.1 hypothetical protein -
H4I82_RS20160 4048032..4048502 + 471 WP_002892026.1 YlaC family protein -
H4I82_RS20165 4048477..4049928 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
H4I82_RS20170 4050029..4050727 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
H4I82_RS20175 4050724..4050864 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
H4I82_RS20180 4050864..4051127 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T173219 WP_002892050.1 NZ_CP061948:4046956-4047174 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T173219 NZ_CP080120:c930380-930003 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT173219 WP_002892066.1 NZ_CP061948:4046555-4046929 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT173219 NZ_CP080120:c930837-930457 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACTCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References