Detailed information of TA system TA173217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2634161..2635749
• Replicon: chromosome
• Accession: NZ_CP061948
• Organism: Klebsiella pneumoniae strain IR5065

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486RAG1
• Locus tag: H4I82_RS13105
• Protein ID: WP_016946382.1
• Coordinates: 2634161..2635483 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: H4I82_RS13110
• Protein ID: WP_004176198.1
• Coordinates: 2635483..2635749 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I82_RS13090	2629217..2631718	+	2502	WP_062920729.1	fimbrial biogenesis outer membrane usher protein	-
H4I82_RS13095	2631706..2632710	+	1005	WP_004184029.1	type 1 fimbrial protein	-
H4I82_RS13100	2632852..2634144	+	1293	WP_062920730.1	glycoside hydrolase family 10 protein	-
H4I82_RS13105	2634161..2635483	-	1323	WP_016946382.1	type II toxin-antitoxin system HipA family toxin	Toxin
H4I82_RS13110	2635483..2635749	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
H4I82_RS13115	2636015..2636341	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
H4I82_RS13120	2636338..2636838	-	501	WP_062920731.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
H4I82_RS13125	2636816..2638195	-	1380	WP_004176204.1	purine permease	-
H4I82_RS13130	2638540..2639694	+	1155	WP_023341545.1	FAD-dependent urate hydroxylase HpxO	-
H4I82_RS13135	2639675..2640598	-	924	WP_062920732.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49125.44 Da
• Isoelectric Point: 7.5358

>T173217 WP_016946382.1 NZ_CP061948:c2635483-2634161 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T173217 NZ_CP080120:749590-749904 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT173217 WP_004176198.1 NZ_CP061948:c2635749-2635483 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT173217 NZ_CP080120:749901-750317 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A486RAG1