Detailed information of TA system TA173205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 3294781..3296411
• Replicon: chromosome
• Accession: NZ_CP061947
• Organism: Algoriphagus sp. Y33

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: -
• Locus tag: ID165_RS13225
• Protein ID: WP_192085311.1
• Coordinates: 3294781..3296082 (-)
• Length: 434 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: ID165_RS13230
• Protein ID: WP_192085312.1
• Coordinates: 3296076..3296411 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ID165_RS13210	3291665..3292435	+	771	WP_192085308.1	YdcF family protein	-
ID165_RS13215	3292660..3293544	+	885	WP_192085309.1	DUF72 domain-containing protein	-
ID165_RS13220	3293651..3294772	+	1122	WP_192085310.1	ATP-binding protein	-
ID165_RS13225	3294781..3296082	-	1302	WP_192085311.1	type II toxin-antitoxin system HipA family toxin	Toxin
ID165_RS13230	3296076..3296411	-	336	WP_192085312.1	transcriptional regulator	Antitoxin
ID165_RS13235	3296553..3300434	-	3882	WP_192085313.1	DNA helicase	-
ID165_RS13240	3300435..3301256	-	822	WP_192085314.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (177-407)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-66)

Sequences

Toxin

Protein

• Length: 434 a.a.
• Molecular weight: 48743.69 Da
• Isoelectric Point: 6.8273

>T173205 WP_192085311.1 NZ_CP061947:c3296082-3294781 [Algoriphagus sp. Y33]
MVVAAEIRIWDKLVGAVLWDDSEQLGSFEFHQDFLRSGLDISPVKMPLSQGNRVHAFPELRRARGDAFDTFKGLPGLLAD
MLPDKYGNQLINTWLIQNGRVADSLNPVEQLCFIGKRGVGALEIRPSLRDETIKASSIEIDSLVNIAGKILNSREDFQSN
LTGEERSALSDILKIGTSAGGARAKAVIAYNPKTGKVKSGQVKAPSGFSYWIIKFDGVHDSQFGSTLGYGRVEMAYFLMA
QSAGIEMSECRLFEENGRAHFMTKRFDRHDDGQKIHMQSLCGLRHFDFNQVGDYSYEQVFETMRMLRLSYPEAEQMFIRM
VFNVLARNCDDHTKNFAFLMDKDGKWKLSPAYDICYAYRPGSVWVSAQSLLVNGKREGIQHADFLEVAHKMNIKKPEEKI
DAVVTAIKKWKEFAEEAKVDSELRDGIASTLLV

Nucleotide

• Length: 1302 bp

>T173205 NZ_CP080119:2823385-2823687 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12991.88 Da
• Isoelectric Point: 10.2079

>AT173205 WP_192085312.1 NZ_CP061947:c3296411-3296076 [Algoriphagus sp. Y33]
MDYNQLSDTAILHQLGRFVKHHRMDQNKSQEQLATAAGISRSTLSLLERGEKVNLITLIQVLRVLDKLQWLESFEVKKTI
SPIDYIKLQKKHERQRIRNSDRAAENPPSEW

Nucleotide

• Length: 336 bp

>AT173205 NZ_CP080119:2823723-2824064 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG