Detailed information of TA system TA173201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2481080..2481665
• Replicon: chromosome
• Accession: NZ_CP061943
• Organism: Pseudomonas chlororaphis strain PCM120

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: IF690_RS11365
• Protein ID: WP_202700103.1
• Coordinates: 2481372..2481665 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: IF690_RS11360
• Protein ID: WP_202700102.1
• Coordinates: 2481080..2481370 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF690_RS11350	2478592..2479974	+	1383	WP_202700100.1	efflux transporter outer membrane subunit	-
IF690_RS11355	2480093..2480680	+	588	WP_202700101.1	hypothetical protein	-
IF690_RS11360	2481080..2481370	-	291	WP_202700102.1	putative addiction module antidote protein	Antitoxin
IF690_RS11365	2481372..2481665	-	294	WP_202700103.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IF690_RS11370	2481903..2483297	-	1395	WP_202700104.1	VOC family protein	-
IF690_RS11375	2483560..2484684	+	1125	WP_202700105.1	diguanylate cyclase	-
IF690_RS11380	2484778..2486022	+	1245	WP_202700106.1	M20/M25/M40 family metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-96)

Antitoxin

• Uncharacterized protein HI1420 (5-90)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10735.38 Da
• Isoelectric Point: 10.8773

>T173201 WP_202700103.1 NZ_CP061943:c2481665-2481372 [Pseudomonas chlororaphis]
MDYEILQTTVFARWHITLRDLRARIAIARRIERASAGNLGDVKNLGGGLSQKRVNTGAGYRLYFTVRGNTLIVLLLGGDK
SSQPADICQARSLAKEF

Nucleotide

• Length: 294 bp

>T173201 NZ_CP080119:2188577-2188864 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10253.80 Da
• Isoelectric Point: 8.0096

>AT173201 WP_202700102.1 NZ_CP061943:c2481370-2481080 [Pseudomonas chlororaphis]
MSDTLVPFDIAALLDSDQAISEYLSQILADGDTDELIRALGHVARARGMTQIAKTSGQGRASLYKAFAPGAKPRFDTVVK
IMRALGLQLCTTVSRG

Nucleotide

• Length: 291 bp

>AT173201 NZ_CP080119:2188851-2189153 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA