Detailed information of TA system TA173193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 513679..514316
• Replicon: chromosome
• Accession: NZ_CP061938
• Organism: Bacillus velezensis strain FIO1408

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: IEN91_RS02480
• Protein ID: WP_003156187.1
• Coordinates: 513966..514316 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: IEN91_RS02475
• Protein ID: WP_003156188.1
• Coordinates: 513679..513960 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IEN91_RS02455	510044..510643	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
IEN91_RS02460	510736..511101	+	366	WP_003156192.1	holo-ACP synthase	-
IEN91_RS02465	511266..512273	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
IEN91_RS02470	512390..513559	+	1170	WP_003156189.1	alanine racemase	-
IEN91_RS02475	513679..513960	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
IEN91_RS02480	513966..514316	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
IEN91_RS02485	514434..515255	+	822	WP_003156182.1	STAS domain-containing protein	-
IEN91_RS02490	515260..515625	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
IEN91_RS02495	515628..516029	+	402	WP_003156178.1	anti-sigma regulatory factor	-
IEN91_RS02500	516041..517048	+	1008	WP_025853725.1	PP2C family protein-serine/threonine phosphatase	-
IEN91_RS02505	517112..517441	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
IEN91_RS02510	517438..517920	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
IEN91_RS02515	517886..518674	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
IEN91_RS02520	518674..519276	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T173193 WP_003156187.1 NZ_CP061938:513966-514316 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T173193 NZ_CP080119:c995333-994806 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT173193 WP_003156188.1 NZ_CP061938:513679-513960 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT173193 NZ_CP080119:c995606-995340 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5