173192

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 139405..140141 Replicon plasmid pBD-50-Km_2
Accession NZ_CP061932
Organism Klebsiella michiganensis strain BD-50-Km

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag IE970_RS32120 Protein ID WP_004098919.1
Coordinates 139659..140141 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A2J5Q9C0
Locus tag IE970_RS32115 Protein ID WP_004098921.1
Coordinates 139405..139671 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE970_RS32070 134588..135589 + 1002 WP_009654304.1 Abi family protein -
IE970_RS32075 135728..136306 - 579 WP_009654302.1 hypothetical protein -
IE970_RS32080 136596..136859 + 264 WP_009310051.1 hypothetical protein -
IE970_RS32085 136856..137422 + 567 WP_009654312.1 hypothetical protein -
IE970_RS32090 137453..137947 + 495 WP_013023776.1 hypothetical protein -
IE970_RS32095 137991..138359 + 369 WP_042946273.1 hypothetical protein -
IE970_RS32100 138390..138593 + 204 WP_004098931.1 hemolysin expression modulator Hha -
IE970_RS32105 138642..138899 + 258 WP_004098928.1 hypothetical protein -
IE970_RS32110 138975..139229 + 255 WP_004118702.1 hypothetical protein -
IE970_RS32115 139405..139671 + 267 WP_004098921.1 DUF1778 domain-containing protein Antitoxin
IE970_RS32120 139659..140141 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
IE970_RS32125 140342..141745 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
IE970_RS32130 141774..142405 - 632 Protein_166 hypothetical protein -
IE970_RS32135 142632..143978 + 1347 WP_077255298.1 ISNCY family transposase -
IE970_RS32140 144037..144828 + 792 WP_003032641.1 tetratricopeptide repeat protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..202348 202348


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T173192 WP_004098919.1 NZ_CP061932:139659-140141 [Klebsiella michiganensis]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T173192 NZ_CP080119:683902-684366 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10105.49 Da        Isoelectric Point: 4.5141

>AT173192 WP_004098921.1 NZ_CP061932:139405-139671 [Klebsiella michiganensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT173192 NZ_CP080119:683567-683902 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A2J5Q9C0

References