Detailed information of TA system TA173191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 92324..92859
• Replicon: plasmid pBD-50-Km_2
• Accession: NZ_CP061932
• Organism: Klebsiella michiganensis strain BD-50-Km

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2N4Z1S3
• Locus tag: IE970_RS31855
• Protein ID: WP_020317119.1
• Coordinates: 92572..92859 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N4Z1S6
• Locus tag: IE970_RS31850
• Protein ID: WP_020317117.1
• Coordinates: 92324..92575 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE970_RS31825	87753..88178	-	426	WP_020317125.1	antirestriction protein	-
IE970_RS31830	88697..88927	-	231	WP_020317122.1	hypothetical protein	-
IE970_RS31835	89182..90402	+	1221	WP_040026172.1	ISL3 family transposase	-
IE970_RS31840	90464..90889	+	426	WP_001776124.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
IE970_RS31845	90889..92160	+	1272	WP_020317121.1	Y-family DNA polymerase	-
IE970_RS31850	92324..92575	+	252	WP_020317117.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IE970_RS31855	92572..92859	+	288	WP_020317119.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IE970_RS31860	92863..93033	+	171	WP_020317124.1	hypothetical protein	-
IE970_RS31865	93036..93311	+	276	WP_020317126.1	hypothetical protein	-
IE970_RS31870	93535..94602	-	1068	WP_020317116.1	hypothetical protein	-
IE970_RS31875	95016..95408	-	393	WP_020317129.1	plasmid partitioning/stability family protein	-
IE970_RS31880	95412..96386	-	975	WP_020317120.1	StbA protein	-
IE970_RS31885	96626..97000	-	375	WP_017901448.1	hypothetical protein	-
IE970_RS31890	97000..97632	-	633	WP_017901447.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..202348	202348
	-	flank	IS/Tn	-	-	89182..90402	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11149.12 Da
• Isoelectric Point: 10.2246

>T173191 WP_020317119.1 NZ_CP061932:92572-92859 [Klebsiella michiganensis]
MTYTVKFREDALKEWQKLDKSIQQQFAKKLKKCCDEPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T173191 NZ_CP080119:c642586-642272 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9200.51 Da
• Isoelectric Point: 3.9507

>AT173191 WP_020317117.1 NZ_CP061932:92324-92575 [Klebsiella michiganensis]
MPNIILSDTSASISELKKNPMATVSAGDGFPVAILNRNQPAFYCIPAELYEKMLDALDDQELAKLVLERSNQQLLDVDLD
TYL

Nucleotide

• Length: 252 bp

>AT173191 NZ_CP080119:c642275-641859 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A2N4Z1S3

Antitoxin

• AlphaFold DB: A0A2N4Z1S6