Detailed information of TA system TA173181

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3865983..3866755
• Replicon: chromosome
• Accession: NZ_CP061930
• Organism: Klebsiella michiganensis strain BD-50-Km

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2I8S4E2
• Locus tag: IE970_RS18385
• Protein ID: WP_065805889.1
• Coordinates: 3866366..3866755 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IE970_RS18380
• Protein ID: WP_175364756.1
• Coordinates: 3865983..3866309 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE970_RS18350	3862428..3863060	-	633	WP_072032909.1	inovirus Gp2 family protein	-
IE970_RS18355	3863598..3864422	+	825	WP_065805884.1	DUF945 domain-containing protein	-
IE970_RS18360	3864419..3864676	+	258	WP_044349282.1	DUF905 domain-containing protein	-
IE970_RS18365	3864756..3865199	+	444	WP_065805885.1	antirestriction protein	-
IE970_RS18370	3865211..3865690	+	480	WP_065805886.1	DNA repair protein RadC	-
IE970_RS18375	3865704..3865925	+	222	WP_065805887.1	DUF987 domain-containing protein	-
IE970_RS18380	3865983..3866309	+	327	WP_175364756.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IE970_RS18385	3866366..3866755	+	390	WP_065805889.1	TA system toxin CbtA family protein	Toxin
IE970_RS18390	3866842..3867687	+	846	WP_065805890.1	DUF4942 domain-containing protein	-
IE970_RS18395	3867857..3867997	-	141	Protein_3590	integrase	-
IE970_RS18400	3868330..3868944	+	615	WP_042944480.1	FMN-binding negative transcriptional regulator	-
IE970_RS18405	3869167..3871143	-	1977	WP_200518521.1	glycoside hydrolase family 28 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3827887..3868944	41057

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14321.40 Da
• Isoelectric Point: 9.9883

>T173181 WP_065805889.1 NZ_CP061930:3866366-3866755 [Klebsiella michiganensis]
MQTKSSPLMRAASSRPSPVSVWQTLLTSLLAQHYGLTLSDTPFSDEQVIQQHIDAGISLVDALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Nucleotide

• Length: 390 bp

>T173181 NZ_CP080118:3260090-3261412 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCAGCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12054.78 Da
• Isoelectric Point: 6.9459

>AT173181 WP_175364756.1 NZ_CP061930:3865983-3866309 [Klebsiella michiganensis]
ISEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANVSGQFCDADLRHLDQAFPILLKQLELMLTSGELNPRHQHCVTLYA
KGLTCEADTLGSHGYVYIAIYPTPVATA

Nucleotide

• Length: 327 bp

>AT173181 NZ_CP080118:3259824-3260090 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A2I8S4E2