Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3748878..3749497 Replicon chromosome
Accession NZ_CP061930
Organism Klebsiella michiganensis strain BD-50-Km

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag IE970_RS17825 Protein ID WP_004099646.1
Coordinates 3748878..3749096 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag IE970_RS17830 Protein ID WP_004129911.1
Coordinates 3749123..3749497 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE970_RS17795 3744923..3745186 + 264 WP_004848323.1 type B 50S ribosomal protein L31 -
IE970_RS17800 3745186..3745326 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
IE970_RS17805 3745323..3746021 - 699 WP_014228203.1 GNAT family N-acetyltransferase -
IE970_RS17810 3746123..3747577 + 1455 WP_200519868.1 PLP-dependent aminotransferase family protein -
IE970_RS17815 3747552..3748022 - 471 WP_014228205.1 YlaC family protein -
IE970_RS17820 3748149..3748715 - 567 WP_038424164.1 maltose O-acetyltransferase -
IE970_RS17825 3748878..3749096 - 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
IE970_RS17830 3749123..3749497 - 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
IE970_RS17835 3749978..3753124 - 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit -
IE970_RS17840 3753147..3754340 - 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T173180 WP_004099646.1 NZ_CP061930:c3749096-3748878 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T173180 NZ_CP061930:c3749096-3748878 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT173180 WP_004129911.1 NZ_CP061930:c3749497-3749123 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT173180 NZ_CP061930:c3749497-3749123 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References