Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3020273..3020849 Replicon chromosome
Accession NZ_CP061930
Organism Klebsiella michiganensis strain BD-50-Km

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag IE970_RS14435 Protein ID WP_038424021.1
Coordinates 3020273..3020560 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID -
Locus tag IE970_RS14440 Protein ID WP_014837314.1
Coordinates 3020547..3020849 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE970_RS14405 3015606..3016124 - 519 WP_004098240.1 hypothetical protein -
IE970_RS14410 3016121..3016921 - 801 WP_046878031.1 winged helix-turn-helix domain-containing protein -
IE970_RS14415 3017067..3017651 - 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
IE970_RS14420 3017799..3018581 - 783 WP_200519786.1 NAD(P)H-dependent oxidoreductase -
IE970_RS14425 3018669..3019577 + 909 WP_200519787.1 LysR family transcriptional regulator -
IE970_RS14430 3019571..3020014 + 444 WP_014837312.1 FosA family fosfomycin resistance glutathione transferase -
IE970_RS14435 3020273..3020560 + 288 WP_038424021.1 BrnT family toxin Toxin
IE970_RS14440 3020547..3020849 + 303 WP_014837314.1 BrnA antitoxin family protein Antitoxin
IE970_RS14445 3020970..3022313 - 1344 WP_049101852.1 APC family permease -
IE970_RS14450 3022310..3023221 - 912 WP_200519788.1 acetamidase/formamidase family protein -
IE970_RS14455 3023675..3024010 - 336 WP_032719937.1 endoribonuclease SymE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(39-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11253.72 Da        Isoelectric Point: 8.5899

>T173178 WP_038424021.1 NZ_CP061930:3020273-3020560 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

>T173178 NZ_CP080118:2667583-2667837 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11549.14 Da        Isoelectric Point: 9.7962

>AT173178 WP_014837314.1 NZ_CP061930:3020547-3020849 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSGIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT173178 NZ_CP080118:2667335-2667586 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References