Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2233736..2234283 Replicon chromosome
Accession NZ_CP061930
Organism Klebsiella michiganensis strain BD-50-Km

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag IE970_RS10775 Protein ID WP_014227299.1
Coordinates 2233975..2234283 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag IE970_RS10770 Protein ID WP_014227298.1
Coordinates 2233736..2233972 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE970_RS10755 2229943..2231109 + 1167 WP_014839828.1 multidrug effflux MFS transporter -
IE970_RS10760 2231270..2232172 + 903 WP_172868716.1 N-acetylmuramic acid 6-phosphate etherase -
IE970_RS10765 2232197..2233630 + 1434 WP_200519659.1 PTS N-acetylmuramic acid transporter subunit IIBC -
IE970_RS10770 2233736..2233972 + 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
IE970_RS10775 2233975..2234283 + 309 WP_014227299.1 CcdB family protein Toxin
IE970_RS10780 2234434..2235336 + 903 WP_014227300.1 EamA family transporter -
IE970_RS10785 2235340..2235597 - 258 WP_042946138.1 hypothetical protein -
IE970_RS10790 2235597..2236574 - 978 WP_032695133.1 Rpn family recombination-promoting nuclease/putative transposase -
IE970_RS10795 2236784..2237077 + 294 WP_014227302.1 hypothetical protein -
IE970_RS10800 2237101..2238042 - 942 WP_014227303.1 LacI family transcriptional regulator -
IE970_RS10805 2238337..2238648 + 312 WP_014227304.1 PTS sugar transporter subunit IIB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T173176 WP_014227299.1 NZ_CP061930:2233975-2234283 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T173176 NZ_CP080118:1745582-1745989 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT173176 WP_014227298.1 NZ_CP061930:2233736-2233972 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT173176 NZ_CP080118:1745335-1745601 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References