Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1484402..1485177 Replicon chromosome
Accession NZ_CP061930
Organism Klebsiella michiganensis strain BD-50-Km

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A2J5Q8N8
Locus tag IE970_RS07155 Protein ID WP_049101740.1
Coordinates 1484402..1484887 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A2J5Q8I3
Locus tag IE970_RS07160 Protein ID WP_049101739.1
Coordinates 1484884..1485177 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE970_RS07150 1483438..1483689 + 252 WP_071846062.1 hypothetical protein -
IE970_RS07155 1484402..1484887 - 486 WP_049101740.1 GNAT family N-acetyltransferase Toxin
IE970_RS07160 1484884..1485177 - 294 WP_049101739.1 DUF1778 domain-containing protein Antitoxin
IE970_RS07165 1485561..1486949 - 1389 WP_032720259.1 DASS family sodium-coupled anion symporter -
IE970_RS07170 1487496..1488866 + 1371 WP_154924412.1 cystathionine beta-synthase -
IE970_RS07175 1488877..1490022 + 1146 WP_038423636.1 PLP-dependent aspartate aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-143)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17553.48 Da        Isoelectric Point: 8.1567

>T173175 WP_049101740.1 NZ_CP061930:c1484887-1484402 [Klebsiella michiganensis]
MISTPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQLSGASRTFVCCHDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDRSLHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMMLMVTLGDLVN
A

Download         Length: 486 bp

>T173175 NZ_CP080118:c1599195-1598818 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10902.55 Da        Isoelectric Point: 9.8590

>AT173175 WP_049101739.1 NZ_CP061930:c1485177-1484884 [Klebsiella michiganensis]
MPAANSMAMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEK

Download         Length: 294 bp

>AT173175 NZ_CP080118:c1599652-1599272 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACTCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q8N8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J5Q8I3

References