Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1337029..1337686 Replicon chromosome
Accession NZ_CP061930
Organism Klebsiella michiganensis strain BD-50-Km

Toxin (Protein)


Gene name cptA Uniprot ID J5U333
Locus tag IE970_RS06395 Protein ID WP_004854060.1
Coordinates 1337029..1337439 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag IE970_RS06400 Protein ID WP_004124953.1
Coordinates 1337420..1337686 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE970_RS06375 1333006..1334739 - 1734 WP_014226791.1 single-stranded-DNA-specific exonuclease RecJ -
IE970_RS06380 1334745..1335458 - 714 WP_004854054.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IE970_RS06385 1335481..1336377 - 897 WP_004105555.1 site-specific tyrosine recombinase XerD -
IE970_RS06390 1336499..1337020 + 522 WP_200519529.1 flavodoxin FldB -
IE970_RS06395 1337029..1337439 - 411 WP_004854060.1 protein YgfX Toxin
IE970_RS06400 1337420..1337686 - 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
IE970_RS06405 1337931..1338914 + 984 WP_014226793.1 tRNA-modifying protein YgfZ -
IE970_RS06410 1339106..1341904 + 2799 WP_200519531.1 sigma-54-dependent transcriptional regulator -
IE970_RS06415 1342025..1342450 + 426 WP_032720093.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16106.97 Da        Isoelectric Point: 10.9455

>T173174 WP_004854060.1 NZ_CP061930:c1337439-1337029 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T173174 NZ_CP080118:c1596732-1595839 [Escherichia coli]
ATGCCTGAATTAACATCATTATTTCCTGACGTTGCCGATGCACTGGATATTGAATCTGTCGCTATCGTCGAAAAAGATTA
TTTTGTGGTTGATCTTTTAAGATTATTAAAAGAAATAAAACCAGAAACACATACTCTCGTGTTTGCAGGTAGTACCGCAT
TATCAAAAGCAGGTATCTCGCTAAACAGAATGTCAGAAGATATTGATATAAAGCTCGTGCCAACAGAGAACTTTATGCAA
AATGGCAGAGACAAGAGAAGAAAAATTCGCAAGGAAATCGTGCAAATAATCACGGATGTTATTCATAATTCTGACATTTT
CAGCTTGGATAATGAAAACGCCCGTATAACACGTGATGAGTATCGTTATAACGAAATCTCGGTACGTTATCCCCAGACAT
TTGCACAAGTCCCCTGTCTTCGTCCATTTATTAAGCTGGAGTTGATGGAAAGCACTCTGCTGGAACATCCGGAAAGCAGA
GATATTTATTCCCTTGTAACGGAGTTAACGGGTAAAGGTACACCTATAACAGCATTCCCCTGTGCCACCATTTTATCCAC
TCAGGCTGAAAAGCTCATCTCCATGATGCGAAGAACAGCAGCACATCTTCGTAATCCCGAACAACAGGATGATGAGTTTC
TGGTACGTCACATTTATGACAACTACTGCATTGTCAGGGAAAAAGGTGTGAATGTTCCTGTATTGAAAAATTTTGTTCAG
ATATGTATTCAACAGGATATTGAACGCTATGGTAACCAGTACCCACAATTTTGTAGCTCACCAGTTGATGAACTTAAGAA
AGGGCTGGATGCACTCCGGGATTATCCTGTACACAAATTGCGCTTTCAACGATTTGTTAACCCGATGGTATTTGGTAATA
CACAGATTAACTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT173174 WP_004124953.1 NZ_CP061930:c1337686-1337420 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT173174 NZ_CP080118:c1597120-1596725 [Escherichia coli]
ATGACTACAAAAGACCGTATTCAGTCCCGACTGAACCGTTCAAAACGTTATGTGTTCACCCGGGATGATTTCAGGGATAT
CGCTGGCTATGATCAGGTTGGCAGAGCTCTTCGTACACTGGTAAACGAAGGTAAGTTGATGAAAGTGGGCTATGGTGTTT
ACACCAAAGCCCGTCGTAACATCATCACTGGGAAAATCATGCCCGCAGCGCCTGGGGGCTCTGCTGCAGTAATTACAGAG
GCCCTGGAGCGCCTGAATATCAGATACCGTCTGGCAGGAGCAACGGAATGCTACAATAAAGGTCAGTCCACACAAATTCC
TGTATCTCTGGGGATAAAAACACCTTCTCGATTTAAAAGAGTGTTAATAGTAGGGAAAAGTAAAATCAATGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References