Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 103866..104456 Replicon chromosome
Accession NZ_CP061930
Organism Klebsiella michiganensis strain BD-50-Km

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag IE970_RS00535 Protein ID WP_014229979.1
Coordinates 103866..104198 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag IE970_RS00540 Protein ID WP_004852307.1
Coordinates 104199..104456 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE970_RS00500 99249..99743 + 495 WP_032693520.1 threonine transporter -
IE970_RS00505 99740..101620 + 1881 WP_032693503.1 AAA family ATPase -
IE970_RS00510 101689..101832 + 144 WP_158499682.1 hypothetical protein -
IE970_RS00515 101991..103010 - 1020 WP_032693504.1 DUF4917 family protein -
IE970_RS00520 103211..103360 - 150 WP_158657239.1 hypothetical protein -
IE970_RS00525 103534..103632 + 99 Protein_102 IS3 family transposase -
IE970_RS00530 103672..103869 + 198 WP_200519831.1 hypothetical protein -
IE970_RS00535 103866..104198 - 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IE970_RS00540 104199..104456 - 258 WP_004852307.1 antitoxin Antitoxin
IE970_RS00545 105178..105285 + 108 Protein_106 molybdopterin-binding protein -
IE970_RS00550 105445..106359 - 915 WP_200519198.1 LysR family transcriptional regulator -
IE970_RS00555 106460..107458 + 999 WP_200519199.1 aldo/keto reductase -
IE970_RS00560 107496..108422 - 927 WP_200519200.1 LysR family transcriptional regulator -
IE970_RS00565 108599..109207 + 609 WP_057174013.1 glutathione S-transferase N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T173173 WP_014229979.1 NZ_CP061930:c104198-103866 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T173173 NZ_CP080118:1419441-1419755 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT173173 WP_004852307.1 NZ_CP061930:c104456-104199 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT173173 NZ_CP080118:1419752-1420168 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References