Detailed information of TA system TA173173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 103866..104456
• Replicon: chromosome
• Accession: NZ_CP061930
• Organism: Klebsiella michiganensis strain BD-50-Km

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A0H3HDW1
• Locus tag: IE970_RS00535
• Protein ID: WP_014229979.1
• Coordinates: 103866..104198 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: J6I3K7
• Locus tag: IE970_RS00540
• Protein ID: WP_004852307.1
• Coordinates: 104199..104456 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE970_RS00500	99249..99743	+	495	WP_032693520.1	threonine transporter	-
IE970_RS00505	99740..101620	+	1881	WP_032693503.1	AAA family ATPase	-
IE970_RS00510	101689..101832	+	144	WP_158499682.1	hypothetical protein	-
IE970_RS00515	101991..103010	-	1020	WP_032693504.1	DUF4917 family protein	-
IE970_RS00520	103211..103360	-	150	WP_158657239.1	hypothetical protein	-
IE970_RS00525	103534..103632	+	99	Protein_102	IS3 family transposase	-
IE970_RS00530	103672..103869	+	198	WP_200519831.1	hypothetical protein	-
IE970_RS00535	103866..104198	-	333	WP_014229979.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IE970_RS00540	104199..104456	-	258	WP_004852307.1	antitoxin	Antitoxin
IE970_RS00545	105178..105285	+	108	Protein_106	molybdopterin-binding protein	-
IE970_RS00550	105445..106359	-	915	WP_200519198.1	LysR family transcriptional regulator	-
IE970_RS00555	106460..107458	+	999	WP_200519199.1	aldo/keto reductase	-
IE970_RS00560	107496..108422	-	927	WP_200519200.1	LysR family transcriptional regulator	-
IE970_RS00565	108599..109207	+	609	WP_057174013.1	glutathione S-transferase N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11740.57 Da
• Isoelectric Point: 10.1863

>T173173 WP_014229979.1 NZ_CP061930:c104198-103866 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T173173 NZ_CP080118:1419441-1419755 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9422.88 Da
• Isoelectric Point: 4.4607

>AT173173 WP_004852307.1 NZ_CP061930:c104456-104199 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT173173 NZ_CP080118:1419752-1420168 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A0H3HDW1

Antitoxin

• AlphaFold DB: A0A0H3HDW8