Detailed information of TA system TA173166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1770..2317
• Replicon: plasmid p1
• Accession: NZ_CP061915
• Organism: Escherichia coli strain SMBL

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: IE857_RS23565
• Protein ID: WP_191596536.1
• Coordinates: 2036..2317 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A3A1Q2G3
• Locus tag: IE857_RS23560
• Protein ID: WP_042021804.1
• Coordinates: 1770..2039 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE857_RS23550	1..858	+	858	WP_126303380.1	incFII family plasmid replication initiator RepA	-
IE857_RS23555	1557..1715	+	159	WP_170994243.1	hypothetical protein	-
IE857_RS23560	1770..2039	+	270	WP_042021804.1	hypothetical protein	Antitoxin
IE857_RS23565	2036..2317	+	282	WP_191596536.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IE857_RS23570	2405..2752	+	348	WP_000142441.1	hypothetical protein	-
IE857_RS23575	2770..3360	-	591	WP_000194574.1	hypothetical protein	-
IE857_RS23580	3360..3617	-	258	WP_001542562.1	hypothetical protein	-
IE857_RS23585	4184..4597	+	414	WP_191596537.1	hypothetical protein	-
IE857_RS23590	4599..5384	+	786	WP_019842589.1	site-specific integrase	-
IE857_RS23595	5968..6600	+	633	WP_000312331.1	ParA family protein	-
IE857_RS23600	6600..6974	+	375	WP_000752652.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..68025	68025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11040.76 Da
• Isoelectric Point: 8.4615

>T173166 WP_191596536.1 NZ_CP061915:2036-2317 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNMMAAIELDERIGYSASSLAWQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T173166 NZ_CP080118:c188089-187916 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10122.48 Da
• Isoelectric Point: 10.2879

>AT173166 WP_042021804.1 NZ_CP061915:1770-2039 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT173166 NZ_CP080118:c188413-188117 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A3A1Q2G3