Detailed information of TA system TA173160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2614527..2615165
• Replicon: chromosome
• Accession: NZ_CP061914
• Organism: Escherichia coli strain SMBL

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: IE857_RS12625
• Protein ID: WP_000813794.1
• Coordinates: 2614989..2615165 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IE857_RS12620
• Protein ID: WP_001270286.1
• Coordinates: 2614527..2614943 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE857_RS12600	2609679..2610620	-	942	WP_001251315.1	ABC transporter permease	-
IE857_RS12605	2610621..2611634	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
IE857_RS12610	2611652..2612797	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
IE857_RS12615	2613042..2614448	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
IE857_RS12620	2614527..2614943	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
IE857_RS12625	2614989..2615165	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
IE857_RS12630	2615387..2615617	+	231	WP_000494244.1	YncJ family protein	-
IE857_RS12635	2615709..2617670	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
IE857_RS12640	2617743..2618279	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
IE857_RS12645	2618332..2619546	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2619586..2620734	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T173160 WP_000813794.1 NZ_CP061914:c2615165-2614989 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T173160 NZ_CP080117:c4216275-4215988 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT173160 WP_001270286.1 NZ_CP061914:c2614943-2614527 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT173160 NZ_CP080117:c4216001-4215699 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• PDB: 6HPB