Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1474168..1474793 Replicon chromosome
Accession NZ_CP061914
Organism Escherichia coli strain SMBL

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag IE857_RS07110 Protein ID WP_000911329.1
Coordinates 1474395..1474793 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag IE857_RS07105 Protein ID WP_000450524.1
Coordinates 1474168..1474395 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE857_RS07080 1469970..1470440 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
IE857_RS07085 1470440..1471012 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
IE857_RS07090 1471158..1472036 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
IE857_RS07095 1472053..1473087 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
IE857_RS07100 1473300..1474013 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
IE857_RS07105 1474168..1474395 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
IE857_RS07110 1474395..1474793 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
IE857_RS07115 1474940..1475803 + 864 WP_001267498.1 neutral zinc metallopeptidase -
IE857_RS07120 1475818..1477833 + 2016 WP_191596462.1 tRNA cytosine(34) acetyltransferase TmcA -
IE857_RS07125 1477907..1478605 + 699 WP_000679823.1 esterase -
IE857_RS07130 1478715..1478915 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T173149 WP_000911329.1 NZ_CP061914:1474395-1474793 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T173149 NZ_CP080117:988409-988816 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT173149 WP_000450524.1 NZ_CP061914:1474168-1474395 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT173149 NZ_CP080117:988162-988428 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References