Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 286745..287376 Replicon chromosome
Accession NZ_CP061914
Organism Escherichia coli strain SMBL

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag IE857_RS01290 Protein ID WP_001259388.1
Coordinates 286745..287020 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag IE857_RS01295 Protein ID WP_000593555.1
Coordinates 287017..287376 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE857_RS01275 281749..282816 + 1068 WP_000361467.1 HlyD family secretion protein -
IE857_RS01280 282813..285548 + 2736 WP_000149124.1 ribosome-associated ATPase/putative transporter RbbA -
IE857_RS01285 285548..286672 + 1125 WP_001216257.1 ABC transporter permease -
IE857_RS01290 286745..287020 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
IE857_RS01295 287017..287376 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IE857_RS01300 287496..287897 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
IE857_RS01305 287903..288709 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
IE857_RS01310 288706..289470 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
IE857_RS01315 289470..290303 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
IE857_RS01320 290300..291244 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 279761..287897 8136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T173144 WP_001259388.1 NZ_CP061914:286745-287020 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T173144 NZ_CP080117:295088-295195 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT173144 WP_000593555.1 NZ_CP061914:287017-287376 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT173144 NZ_CP080117:c295039-294974 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References