Detailed information of TA system TA173143

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 50952..51743
• Replicon: chromosome
• Accession: NZ_CP061914
• Organism: Escherichia coli strain SMBL

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: IE857_RS00250
• Protein ID: WP_000691787.1
• Coordinates: 50952..51377 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A775TFQ7
• Locus tag: IE857_RS00255
• Protein ID: WP_000939440.1
• Coordinates: 51408..51743 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE857_RS00230	47797..48183	-	387	WP_001270938.1	DUF2441 domain-containing protein	-
IE857_RS00235	48806..49537	-	732	WP_001216640.1	TIGR02646 family protein	-
IE857_RS00240	49534..49755	-	222	WP_126125326.1	hypothetical protein	-
IE857_RS00245	49740..50931	-	1192	Protein_48	AAA family ATPase	-
IE857_RS00250	50952..51377	-	426	WP_000691787.1	TA system toxin CbtA family protein	Toxin
IE857_RS00255	51408..51743	-	336	WP_000939440.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IE857_RS00260	51743..52216	-	474	WP_191596386.1	DNA repair protein RadC	-
IE857_RS00265	52246..53064	-	819	WP_001046748.1	DUF945 domain-containing protein	-
IE857_RS00270	53301..54254	-	954	WP_000290409.1	hypothetical protein	-
IE857_RS00275	54847..55461	+	615	WP_000772911.1	inovirus Gp2 family protein	-
IE857_RS00280	55579..55797	+	219	WP_001064742.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16564.27 Da
• Isoelectric Point: 10.2369

>T173143 WP_000691787.1 NZ_CP061914:c51377-50952 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQILLARLLEQHYGLNLNDTPFNNEKVIQERIDAGITLVDAVNFLVEKYELIRIDRKVF
SWQEQTPYLRAVDVLRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T173143 NZ_CP080117:c88437-88060 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12586.58 Da
• Isoelectric Point: 7.6977

>AT173143 WP_000939440.1 NZ_CP061914:c51743-51408 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQTFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT173143 NZ_CP080117:c88895-88527 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A775TFQ7