173128

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 786879..787515 Replicon chromosome
Accession NZ_CP061870
Organism Bacillus subtilis strain FX-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag IE382_RS04065 Protein ID WP_003156187.1
Coordinates 787165..787515 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag IE382_RS04060 Protein ID WP_003225183.1
Coordinates 786879..787160 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE382_RS04040 (783239) 783239..783838 - 600 WP_041351790.1 rhomboid family intramembrane serine protease -
IE382_RS04045 (783933) 783933..784298 + 366 WP_014475874.1 holo-ACP synthase -
IE382_RS04050 (784464) 784464..785480 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
IE382_RS04055 (785594) 785594..786763 + 1170 WP_017697002.1 alanine racemase -
IE382_RS04060 (786879) 786879..787160 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
IE382_RS04065 (787165) 787165..787515 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IE382_RS04070 (787630) 787630..788454 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
IE382_RS04075 (788459) 788459..788824 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
IE382_RS04080 (788828) 788828..789229 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
IE382_RS04085 (789241) 789241..790248 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
IE382_RS04090 (790310) 790310..790639 + 330 WP_014662855.1 anti-sigma factor antagonist RsbV -
IE382_RS04095 (790636) 790636..791118 + 483 WP_003234299.1 anti-sigma B factor RsbW -
IE382_RS04100 (791084) 791084..791872 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
IE382_RS04105 (791872) 791872..792471 + 600 WP_017696999.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T173128 WP_003156187.1 NZ_CP061870:787165-787515 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T173128 NZ_CP080116:c1819212-1819039 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT173128 WP_003225183.1 NZ_CP061870:786879-787160 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT173128 NZ_CP080116:c1819536-1819240 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References