Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 207748..208385 Replicon chromosome
Accession NZ_CP061868
Organism Priestia koreensis strain FS-1

Toxin (Protein)


Gene name mazF Uniprot ID A0A0M0KPQ0
Locus tag IE339_RS01175 Protein ID WP_053403428.1
Coordinates 208035..208385 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0M0KNK0
Locus tag IE339_RS01170 Protein ID WP_053403467.1
Coordinates 207748..208029 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE339_RS01150 (IE339_01150) 203779..204744 + 966 WP_242172866.1 UV DNA damage repair endonuclease UvsE -
IE339_RS01155 (IE339_01155) 204788..205153 + 366 WP_242172868.1 holo-ACP synthase -
IE339_RS01160 (IE339_01160) 205269..206273 + 1005 WP_242172872.1 outer membrane lipoprotein carrier protein LolA -
IE339_RS01165 (IE339_01165) 206392..207558 + 1167 WP_242172874.1 alanine racemase -
IE339_RS01170 (IE339_01170) 207748..208029 + 282 WP_053403467.1 YlcI/YnfO family protein Antitoxin
IE339_RS01175 (IE339_01175) 208035..208385 + 351 WP_053403428.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IE339_RS01180 (IE339_01180) 208622..209455 + 834 WP_242172876.1 STAS domain-containing protein -
IE339_RS01185 (IE339_01185) 209452..209808 + 357 WP_053403426.1 STAS domain-containing protein -
IE339_RS01190 (IE339_01190) 209812..210213 + 402 WP_242172878.1 anti-sigma regulatory factor -
IE339_RS01195 (IE339_01195) 210227..211237 + 1011 WP_242172880.1 PP2C family protein-serine/threonine phosphatase -
IE339_RS01200 (IE339_01200) 211296..211628 + 333 WP_242172883.1 STAS domain-containing protein -
IE339_RS01205 (IE339_01205) 211625..212110 + 486 WP_053403422.1 anti-sigma B factor RsbW -
IE339_RS01210 (IE339_01210) 212076..212870 + 795 WP_242172885.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13019.08 Da        Isoelectric Point: 5.7284

>T173127 WP_053403428.1 NZ_CP061868:208035-208385 [Priestia koreensis]
MVVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDEEMMDRVDKALQVSLGLIDF

Download         Length: 351 bp

>T173127 NZ_CP080116:c1716288-1715938 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10720.32 Da        Isoelectric Point: 7.1654

>AT173127 WP_053403467.1 NZ_CP061868:207748-208029 [Priestia koreensis]
VSEVSATTEILVRLPQSLVSELDVLVKQENGNRSDFIYQATKMYLRERKKRHIRETMKRGYMEMAKINLNIACEAFLAEY
EADHTVERLVSGG

Download         Length: 282 bp

>AT173127 NZ_CP080116:c1716533-1716282 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M0KPQ0

Antitoxin

Source ID Structure
AlphaFold DB A0A0M0KNK0

References