Detailed information of TA system TA173125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1252454..1253371
• Replicon: chromosome
• Accession: NZ_CP061853
• Organism: Bacillus amyloliquefaciens strain MOH1-5b

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: BAMOH1_RS06475
• Protein ID: WP_007407256.1
• Coordinates: 1252625..1253371 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: BAMOH1_RS06470
• Protein ID: WP_003154807.1
• Coordinates: 1252454..1252624 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAMOH1_RS06435	1247687..1249309	+	1623	WP_072177386.1	pyocin knob domain-containing protein	-
BAMOH1_RS06440	1249322..1249693	+	372	WP_017417608.1	hypothetical protein	-
BAMOH1_RS06445	1249698..1249895	+	198	WP_007610833.1	XkdX family protein	-
BAMOH1_RS06450	1249952..1250713	+	762	WP_094295696.1	phage portal protein	-
BAMOH1_RS06455	1250765..1251028	+	264	WP_021493821.1	hemolysin XhlA family protein	-
BAMOH1_RS06460	1251042..1251305	+	264	WP_057079949.1	phage holin	-
BAMOH1_RS06465	1251319..1252197	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
BAMOH1_RS06470	1252454..1252624	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BAMOH1_RS06475	1252625..1253371	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BAMOH1_RS06480	1253476..1254474	-	999	WP_003154805.1	inorganic phosphate transporter	-
BAMOH1_RS06485	1254487..1255104	-	618	WP_003154804.1	DUF47 domain-containing protein	-
BAMOH1_RS06490	1255390..1256706	-	1317	WP_007610842.1	amino acid permease	-
BAMOH1_RS06495	1257028..1257978	+	951	WP_014417529.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T173125 WP_007407256.1 NZ_CP061853:c1253371-1252625 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T173125 NZ_CP080116:c1523520-1523233 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT173125 WP_003154807.1 NZ_CP061853:c1252624-1252454 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT173125 NZ_CP080116:c1523246-1522944 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14