Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 480100..480737 Replicon chromosome
Accession NZ_CP061853
Organism Bacillus amyloliquefaciens strain MOH1-5b

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BAMOH1_RS02410 Protein ID WP_003156187.1
Coordinates 480387..480737 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BAMOH1_RS02405 Protein ID WP_003156188.1
Coordinates 480100..480381 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAMOH1_RS02385 476465..477064 - 600 WP_053573675.1 rhomboid family intramembrane serine protease -
BAMOH1_RS02390 477157..477522 + 366 WP_014304402.1 holo-ACP synthase -
BAMOH1_RS02395 477687..478694 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
BAMOH1_RS02400 478811..479980 + 1170 WP_021495079.1 alanine racemase -
BAMOH1_RS02405 480100..480381 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BAMOH1_RS02410 480387..480737 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BAMOH1_RS02415 480855..481676 + 822 WP_003156182.1 STAS domain-containing protein -
BAMOH1_RS02420 481681..482046 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
BAMOH1_RS02425 482049..482450 + 402 WP_003156178.1 anti-sigma regulatory factor -
BAMOH1_RS02430 482462..483469 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
BAMOH1_RS02435 483533..483862 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
BAMOH1_RS02440 483859..484341 + 483 WP_007609591.1 anti-sigma B factor RsbW -
BAMOH1_RS02445 484307..485095 + 789 WP_029974100.1 RNA polymerase sigma factor SigB -
BAMOH1_RS02450 485095..485697 + 603 WP_128496463.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T173124 WP_003156187.1 NZ_CP061853:480387-480737 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T173124 NZ_CP080116:c1413242-1412958 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT173124 WP_003156188.1 NZ_CP061853:480100-480381 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT173124 NZ_CP080116:c1413478-1413245 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References