Detailed information of TA system TA173122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 521546..522183
• Replicon: chromosome
• Accession: NZ_CP061852
• Organism: Bacillus amyloliquefaciens strain INH2-4b

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BAINH2_RS02460
• Protein ID: WP_003156187.1
• Coordinates: 521833..522183 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BAINH2_RS02455
• Protein ID: WP_003156188.1
• Coordinates: 521546..521827 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAINH2_RS02435	517911..518510	-	600	WP_053573675.1	rhomboid family intramembrane serine protease	-
BAINH2_RS02440	518603..518968	+	366	WP_015416872.1	holo-ACP synthase	-
BAINH2_RS02445	519133..520140	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
BAINH2_RS02450	520257..521426	+	1170	WP_053573676.1	alanine racemase	-
BAINH2_RS02455	521546..521827	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BAINH2_RS02460	521833..522183	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BAINH2_RS02465	522301..523122	+	822	WP_033575045.1	STAS domain-containing protein	-
BAINH2_RS02470	523127..523492	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BAINH2_RS02475	523495..523896	+	402	WP_003156178.1	anti-sigma regulatory factor	-
BAINH2_RS02480	523908..524915	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
BAINH2_RS02485	524979..525308	+	330	WP_033575044.1	anti-sigma factor antagonist	-
BAINH2_RS02490	525305..525787	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
BAINH2_RS02495	525753..526541	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
BAINH2_RS02500	526541..527143	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T173122 WP_003156187.1 NZ_CP061852:521833-522183 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T173122 NZ_CP080116:962446-962664 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT173122 WP_003156188.1 NZ_CP061852:521546-521827 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT173122 NZ_CP080116:962046-962420 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5