Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 521546..522183 Replicon chromosome
Accession NZ_CP061852
Organism Bacillus amyloliquefaciens strain INH2-4b

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BAINH2_RS02460 Protein ID WP_003156187.1
Coordinates 521833..522183 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BAINH2_RS02455 Protein ID WP_003156188.1
Coordinates 521546..521827 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAINH2_RS02435 517911..518510 - 600 WP_053573675.1 rhomboid family intramembrane serine protease -
BAINH2_RS02440 518603..518968 + 366 WP_015416872.1 holo-ACP synthase -
BAINH2_RS02445 519133..520140 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
BAINH2_RS02450 520257..521426 + 1170 WP_053573676.1 alanine racemase -
BAINH2_RS02455 521546..521827 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BAINH2_RS02460 521833..522183 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BAINH2_RS02465 522301..523122 + 822 WP_033575045.1 STAS domain-containing protein -
BAINH2_RS02470 523127..523492 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
BAINH2_RS02475 523495..523896 + 402 WP_003156178.1 anti-sigma regulatory factor -
BAINH2_RS02480 523908..524915 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
BAINH2_RS02485 524979..525308 + 330 WP_033575044.1 anti-sigma factor antagonist -
BAINH2_RS02490 525305..525787 + 483 WP_007609591.1 anti-sigma B factor RsbW -
BAINH2_RS02495 525753..526541 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
BAINH2_RS02500 526541..527143 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T173122 WP_003156187.1 NZ_CP061852:521833-522183 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T173122 NZ_CP080116:962446-962664 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT173122 WP_003156188.1 NZ_CP061852:521546-521827 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT173122 NZ_CP080116:962046-962420 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References