Detailed information of TA system TA173119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4926562..4927148
• Replicon: chromosome
• Accession: NZ_CP061850
• Organism: Pseudomonas aeruginosa strain R31

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: IE752_RS22845
• Protein ID: WP_003120987.1
• Coordinates: 4926849..4927148 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: IE752_RS22840
• Protein ID: WP_003448662.1
• Coordinates: 4926562..4926852 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE752_RS22825	4922144..4924033	+	1890	WP_003448700.1	hypothetical protein	-
IE752_RS22830	4924030..4926006	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
IE752_RS22835	4926147..4926491	+	345	WP_016851612.1	hypothetical protein	-
IE752_RS22840	4926562..4926852	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
IE752_RS22845	4926849..4927148	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IE752_RS22850	4927350..4928474	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
IE752_RS22855	4928485..4930182	+	1698	WP_061195053.1	PilN family type IVB pilus formation outer membrane protein	-
IE752_RS22860	4930186..4931511	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
IE752_RS22865	4931501..4932034	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4894955..4988103	93148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T173119 WP_003120987.1 NZ_CP061850:c4927148-4926849 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T173119 NZ_CP080109:c138232-137747 [Enterobacter asburiae]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGTTGCCATCAACTTGCTGAGTTCGCCAGTGGTGAGACTGTACTTGA
TGACTGGCTTAAACAGAGAGGATTGAAAAACCAGGCTCTGGGGGCTGCCCGAACGTTCGTAGTTTGTAAAGCAAACACGA
AACAGGTTGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAACGGGAAGTCTCAGGCGCAACATG
CCAGATCCTATTCCGGTAATCATACTTGCTCGCCTGGCAGTCGATGTTTCATTCCGGGGAAAGGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTATCGTGTAGCTGAGAATATTGGAGTCCGTGCAATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAGTTTTTATCTTCATCATGGCTTTAAGCCATCGCAAACCCAGGAACGTACACTGTTTCTCAAGTTACCA
CAGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT173119 WP_003448662.1 NZ_CP061850:c4926852-4926562 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT173119 NZ_CP080109:c138486-138220 [Enterobacter asburiae]
ATGAAATCAGATGTTCAGCTCAACCTCAGAGCCAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACAGACTTCATTCTGGAAATGGCATGCCAGGCGGCCGAGAATGTTATCCTCGACCGTCGCGTTTTTAATT
TTAACGATGAGCAGTACGCAGAGTTTATTGATATGCTCGATGCACCAGTTGCGGATAACGTTGCCATTGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: G8CP73