Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4066341..4066993 Replicon chromosome
Accession NZ_CP061850
Organism Pseudomonas aeruginosa strain R31

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag IE752_RS18855 Protein ID WP_003134109.1
Coordinates 4066341..4066751 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag IE752_RS18860 Protein ID WP_170869361.1
Coordinates 4066751..4066993 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE752_RS18810 4061377..4062702 + 1326 WP_191519258.1 hypothetical protein -
IE752_RS18815 4062750..4063102 + 353 Protein_3721 hypothetical protein -
IE752_RS18820 4063014..4063211 + 198 WP_009315864.1 hypothetical protein -
IE752_RS18825 4063368..4064096 + 729 WP_009315862.1 TIGR03761 family integrating conjugative element protein -
IE752_RS18830 4064102..4064632 + 531 WP_121372660.1 DUF3158 family protein -
IE752_RS18835 4064646..4065134 + 489 WP_023104904.1 single-stranded DNA-binding protein -
IE752_RS18840 4065633..4065830 + 198 WP_191519259.1 CrpP family protein -
IE752_RS18845 4065896..4066114 - 219 WP_023083218.1 hypothetical protein -
IE752_RS18850 4066164..4066325 - 162 WP_191519260.1 hypothetical protein -
IE752_RS18855 4066341..4066751 - 411 WP_003134109.1 tRNA(fMet)-specific endonuclease VapC Toxin
IE752_RS18860 4066751..4066993 - 243 WP_170869361.1 antitoxin Antitoxin
IE752_RS18865 4067299..4069224 + 1926 WP_191519261.1 type I DNA topoisomerase -
IE752_RS18870 4069232..4069984 + 753 WP_191519262.1 hypothetical protein -
IE752_RS18875 4070096..4071985 + 1890 WP_191519263.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - katA 4047878..4174686 126808


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(11-37)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 15452.78 Da        Isoelectric Point: 7.3233

>T173118 WP_003134109.1 NZ_CP061850:c4066751-4066341 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Download         Length: 411 bp

>T173118 NZ_CP080109:61064-61351 [Enterobacter asburiae]
ATGGCATATTTTCTGGATTTTGACGAGCGTGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGCTAAA
AAATAAGTTGGCTGAAGTGCTTGAGTCACCCCGAATTGATGCGAACAAGCTTCGGGGTATGCATGACTGTTACAAAATTA
AGCTCCGGTCTTCAGGCTATCGTCTTGTATATCAGGTCATAGACGAGAAGGTTGTCGTCTTTGTGATTTCTGTCGGGAAA
AGAGAGCGCTCGGAAGTTTATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8909.94 Da        Isoelectric Point: 4.4230

>AT173118 WP_170869361.1 NZ_CP061850:c4066993-4066751 [Pseudomonas aeruginosa]
MRCVVEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Download         Length: 243 bp

>AT173118 NZ_CP080109:60825-61064 [Enterobacter asburiae]
ATGGGTAGCATCAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCTGCGCTTGAAAAAATGGGTGTAACTCC
CTCTGAGGCACTTCGCCTCATGCTTGAATATATTGCTGACAATGAACGTTTGCCGTTCAAACAGACACTCCTGAGTGATG
AGGATGCTGAGCTCGTGGAGATCGTGAAAGAACGACTTCGCAATCCTAAGCCAGTACGTGTGACGCTGGATGAGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References