Detailed information of TA system TA173109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2571413..2571929
• Replicon: chromosome
• Accession: NZ_CP061848
• Organism: Pseudomonas asiatica strain C3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F8G1E7
• Locus tag: IE322_RS11645
• Protein ID: WP_003259987.1
• Coordinates: 2571413..2571694 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F8G1E6
• Locus tag: IE322_RS11650
• Protein ID: WP_013972043.1
• Coordinates: 2571684..2571929 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE322_RS11620	2566488..2567729	-	1242	WP_191472873.1	Zn-dependent hydrolase	-
IE322_RS11625	2567760..2569064	-	1305	WP_191472875.1	MHS family MFS transporter	-
IE322_RS11630	2569356..2570264	+	909	WP_085663256.1	LysR family transcriptional regulator	-
IE322_RS11635	2570353..2570748	+	396	WP_023662326.1	hypothetical protein	-
IE322_RS11640	2570765..2571397	+	633	WP_015269938.1	BRCT domain-containing protein	-
IE322_RS11645	2571413..2571694	-	282	WP_003259987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IE322_RS11650	2571684..2571929	-	246	WP_013972043.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IE322_RS11655	2572173..2573279	-	1107	WP_054572951.1	alkene reductase	-
IE322_RS11660	2573310..2574503	-	1194	WP_015269936.1	MFS transporter	-
IE322_RS11665	2574671..2574736	+	66	Protein_2313	short-chain dehydrogenase/reductase SDR	-
IE322_RS11670	2575239..2575781	+	543	WP_015269935.1	WYL domain-containing protein	-
IE322_RS11675	2576038..2576517	+	480	WP_191472877.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10909.78 Da
• Isoelectric Point: 10.7594

>T173109 WP_003259987.1 NZ_CP061848:c2571694-2571413 [Pseudomonas asiatica]
MTYKLEFLPSALKEWGKLGHTVREQIKKKLAERLQSPKVQADALRDLPNHYKIKLKASGYRLVYRVEDERIVVVVVSVGK
RERSEVYRSAQKR

Nucleotide

• Length: 282 bp

>T173109 NZ_CP080107:701959-702246 [Enterobacter asburiae]
ATGCCAACGGAGTTTGAATGGGATACCAACAAAGCGAAAAGCAATCTCATTAAGCATGGGATCCGCTTTGAAGAAGCTGT
TCTGGTGTTTGACGATCCTTACCATTTATCTTTGCAGGATCGTCATGAAAATGGAGAGTTCCGTTGGCAGACCATTGGCC
TTGTACATGGTCTAATTGTCATTATGGTCGCACACACGGTACGTTTTGAAAGTGGTGACGAAGTCATTCGAATCATTAGC
GCACGAAAGGCGGACCGTAAAGAGAGGAGCCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8779.21 Da
• Isoelectric Point: 4.3611

>AT173109 WP_013972043.1 NZ_CP061848:c2571929-2571684 [Pseudomonas asiatica]
MQNIFADVAVSVSELKKNPSAVMTGAAGMPVAVLNHNRVMGYMVPAELYEAMMERLEDLELSELAKSRLGEKGVPVSLDD
L

Nucleotide

• Length: 246 bp

>AT173109 NZ_CP080107:702233-702535 [Enterobacter asburiae]
ATGAGCATGGTTAAACATAAACGCGGTAGTGCGCCTGAGATGAGTGCTCGCCGTGAGGCTGAGCTCAAAGCACTAGCCGA
AAAATCGGAAGCAGAGATTGATTACAGCGATATCCCTGCCACGGGAGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGGCCACTCAAAACTCAGGCGTCTGTACGTATCGACGCTGACGTGATGGAGTGGCTTAAACGGCCTGGAAAAGGT
TACCAGACGAGGCTCAATGCCATTTTGCGTGAAGCTATGCTGCGCGATCAAAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: F8G1E7

Antitoxin

• AlphaFold DB: F8G1E6