Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 1043661..1044700 Replicon chromosome
Accession NZ_CP061848
Organism Pseudomonas asiatica strain C3

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A2N5BP56
Locus tag IE322_RS04630 Protein ID WP_015271072.1
Coordinates 1043661..1044236 (-) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A2N5BP88
Locus tag IE322_RS04635 Protein ID WP_015271071.1
Coordinates 1044233..1044700 (-) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE322_RS04620 1039248..1040153 - 906 WP_013973410.1 LysR family transcriptional regulator -
IE322_RS04625 1040516..1043446 - 2931 WP_137188394.1 aminotransferase -
IE322_RS04630 1043661..1044236 - 576 WP_015271072.1 PIN domain-containing protein Toxin
IE322_RS04635 1044233..1044700 - 468 WP_015271071.1 helix-turn-helix domain-containing protein Antitoxin
IE322_RS04640 1044882..1045145 - 264 WP_015271070.1 DUF3077 domain-containing protein -
IE322_RS04645 1045302..1045544 - 243 WP_015271069.1 hypothetical protein -
IE322_RS04650 1046251..1047864 - 1614 WP_085720482.1 ABC transporter ATP-binding protein -
IE322_RS04655 1047866..1048885 - 1020 WP_013973404.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1034265..1044700 10435


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

SinI-like, DNA-binding domain

(84-129)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21970.23 Da        Isoelectric Point: 5.0798

>T173107 WP_015271072.1 NZ_CP061848:c1044236-1043661 [Pseudomonas asiatica]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSTKIHDEWKRNLLLNRPDLTLEQVDRTSRRMDAAVPDALVTDY
ESLVEGLDLPDKDDRHVLAAAIKCNASVIVTFNLKDFPETALDVFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRMSLKN
PPHSVQQYLDRLLQQKLPESVKLLSEFKFLL

Download         Length: 576 bp

>T173107 NZ_CP080104:8165-8440 [Klebsiella quasipneumoniae]
TTGTCGAAGTGGAATATTAAGTTCACGAAGAATGGCGCTGATGATATCAATTACTGGCGTGGTACTGACAAAAAGAAGTT
TGAACGTATCCGGCAGCTACTTCACAGCATCGAGTCTGATCCGTTAACTGGTATAGGGAAGCCTGAGCGATTACGCCATC
ACAAAGATCCTGCGCTCTATTCTCGGCGTATCGATCAGAGCCATAGGCTTGTATATTCAATCCAAAACGGAGAAATTATC
ATCTACGCAGCGCGTTATCACTACGGCGATAAATAG

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17366.86 Da        Isoelectric Point: 5.3408

>AT173107 WP_015271071.1 NZ_CP061848:c1044700-1044233 [Pseudomonas asiatica]
MTTADLSRKMLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPYLVKLLDESVIPHTKTGRHRRVKFSDLVAYKDKRDEVSRAAMDALAAEAQELRMGYE

Download         Length: 468 bp

>AT173107 NZ_CP080104:7939-8184 [Klebsiella quasipneumoniae]
ATGCATGCAATCACTTTTACGGATGCCCGTAAACATTTTGCCGATACCATGAAGCGTGTTACCGATGATGCTGAGCCAGT
ACGAGTAATGCGGCGAGATGCACCTGATATCGTCATGATCGATGCTGGAGAGTATGAGGCACTCATAGAAACAGTGTATC
TGTTCAGCAATCCTGCGAATGCTGCTCATATCAATGAGTCAATGAAACAGGCGGAGCGCGGCGAATTTGTCGAAGTGGAA
TATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N5BP56

Antitoxin

Source ID Structure
AlphaFold DB A0A2N5BP88

References