Detailed information of TA system TA173103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK(toxin)
• Location: 1035276..1035889
• Replicon: chromosome
• Accession: NZ_CP061846
• Organism: Chryseoglobus sp. 28M-23

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IE160_RS05035
• Protein ID: WP_191151984.1
• Coordinates: 1035545..1035889 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IE160_RS05030
• Protein ID: WP_179873611.1
• Coordinates: 1035276..1035551 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE160_RS05005	1032408..1032716	+	309	WP_152583138.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IE160_RS05010	1032706..1033005	+	300	WP_152583106.1	putative addiction module antidote protein	-
IE160_RS05015	1033092..1033355	+	264	WP_152583105.1	ribbon-helix-helix protein, CopG family	-
IE160_RS05020	1033393..1034706	+	1314	WP_191151821.1	plasmid pRiA4b ORF-3 family protein	-
IE160_RS05025	1034814..1035242	+	429	WP_179873610.1	hypothetical protein	-
IE160_RS05030	1035276..1035551	+	276	WP_179873611.1	hypothetical protein	Antitoxin
IE160_RS05035	1035545..1035889	+	345	WP_191151984.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IE160_RS05040	1035879..1036280	-	402	WP_191151822.1	DUF1622 domain-containing protein	-
IE160_RS05045	1036347..1036571	+	225	Protein_990	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IE160_RS05050	1036727..1037662	-	936	WP_152583099.1	DUF2510 domain-containing protein	-
IE160_RS05055	1037744..1038511	+	768	WP_152583098.1	alpha/beta hydrolase	-
IE160_RS05060	1038581..1038796	-	216	WP_152583097.1	hypothetical protein	-
IE160_RS05065	1038832..1039095	-	264	WP_152583096.1	hypothetical protein	-
IE160_RS05070	1039092..1039388	-	297	WP_191151823.1	hypothetical protein	-
IE160_RS05075	1039497..1040339	+	843	WP_191151824.1	CPBP family intramembrane metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1008722..1036280	27558

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12049.98 Da
• Isoelectric Point: 9.2325

>T173103 WP_191151984.1 NZ_CP061846:1035545-1035889 [Chryseoglobus sp. 28M-23]
MVIARGDVCWVDLGPIVDRGPAKHRPVVIVQSDDYTRSRLGTVVVAAISSSTARAGIPGNVFVPTSASGLPKDSVVIATE
VLTLDRDHIGEPIGRLPTPLMRGIDSGLRRVLSL

Nucleotide

• Length: 345 bp

>T173103 NZ_CP080099:c4962882-4962598 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGCGTTTGATCCGCGTGCCTGGCGCGAATGGCAGAAGCTTGGCGAGACGATCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGGTTGTGCAGAATCCGCGAATTGCATCGGCCAGTCTGAGTGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGGGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10315.36 Da
• Isoelectric Point: 4.4687

>AT173103 WP_179873611.1 NZ_CP061846:1035276-1035551 [Chryseoglobus sp. 28M-23]
MKTAISVPDPLFEKVERARPDLGMSRSQFYATAAKHYLDSVEREALTSEIDAALQTVRGDSHAQAAEEHERADLSQQSLR
RLELLTEDDDW

Nucleotide

• Length: 276 bp

>AT173103 NZ_CP080099:c4963114-4962872 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTGCGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCAGTGAAAAGTATGACTATCAGCGATA
GCGAAGCGGCATTATTACGCACCGTCCGCGAGCGGCTGGAAAACCCGCAGCCGGAGGTGAAGGTGAGTCTGGATGACCTA
TGA