Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 6029..6554 Replicon plasmid pHC139-5copy
Accession NZ_CP061843
Organism Klebsiella pneumoniae strain HC139

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag IEY90_RS24795 Protein ID WP_013023785.1
Coordinates 6029..6334 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag IEY90_RS24800 Protein ID WP_001568025.1
Coordinates 6336..6554 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEY90_RS24765 1756..2382 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
IEY90_RS24770 2502..2681 + 180 WP_006788217.1 hypothetical protein -
IEY90_RS24775 3105..3899 - 795 WP_004197635.1 site-specific integrase -
IEY90_RS24780 4097..5113 - 1017 WP_017899884.1 hypothetical protein -
IEY90_RS24785 5124..5438 - 315 WP_053389906.1 hypothetical protein -
IEY90_RS24790 5465..5860 - 396 WP_017899885.1 hypothetical protein -
IEY90_RS24795 6029..6334 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
IEY90_RS24800 6336..6554 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IEY90_RS24805 7373..7663 - 291 WP_013023783.1 hypothetical protein -
IEY90_RS24810 7660..8787 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
IEY90_RS24815 8821..10414 - 1594 Protein_11 hypothetical protein -
IEY90_RS24820 10621..11401 - 781 Protein_12 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / sul1 / qnrB91 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / aph(3')-Ia / tet(A) / floR / blaTEM-1B / blaCTX-M-3 / qnrS1 - 1..173471 173471


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T173100 WP_013023785.1 NZ_CP061843:c6334-6029 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T173100 NZ_CP080099:c2726705-2725383 [Klebsiella quasipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGACGAGTGGCTGCGCAGCCCGCGCGCCAGGCCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGTTGCCCGACAGCCCACAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAACAGCCGTTCGATCTGCTGGCCGAGGTCGGCCGGGATAGCGTAGGGGCGGTGACTCTGCTGCCGCCGGG
AGAAGAGACGCATCTTGCAGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAACTGACCGCGTTGCTGACCGCCTACCAGT
CGGATATTCCGCTCGGGATGGTGACCGGCCAGGACGATTTTCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACCGCGCTG
CTGCGAATAGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATTGGCGAGAT
AAAACAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATCTGTGCCTGGCGCTGGCCAGGGCGTTGG
ACCTGGCGGTGCCGGAGGCGGAGATTATCACCACCCCGCGGATCCGTGCGCTGGCGGTGACCCGTTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGGCTGCCGCAGGAGGATCTGTGTCAGGCATTCGGCATACCGTCGTCGATGAAATA
CGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTAACCGGGGCAACGGACGGGCATGCGAAGAATTTCTCGCTCTTTTTGCTG
CCCGGCGGCAGCTATCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGAGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAACGCCACCCGAGGGCGGAAAACGGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTTCCCCGGGATCAGATGCTGGCGATCCTTGACGAGTTTGCCGACCGCGTGCCG
CAGGCCATCGAAAGCGCCCGCCGGACGCTGCCGGCGGACTTTTCCGCCCACGTCTGGCGGGCGATCACGGAGAACATGCT
GAAACTGCACGCTCGCCTGCAGCAAGGGCTGCAGGCGCGATAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT173100 WP_001568025.1 NZ_CP061843:c6554-6336 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT173100 NZ_CP080099:c2726971-2726705 [Klebsiella quasipneumoniae]
ATGCATTCCCCGCAGATATACAGTCCCGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCGGACAAAACCACCTTGA
CAACCCTGTTCAAGATCCTCCAGTCGCTGGGGGTGGTGATGACCCTGAACGCCAAACCGGCCGAGACGGCGACGGCCCCC
GATAACACCACGGATCTGGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References