Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5020779..5021404 Replicon chromosome
Accession NZ_CP061842
Organism Klebsiella pneumoniae strain HC139

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IEY90_RS24395 Protein ID WP_002882817.1
Coordinates 5020779..5021162 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IEY90_RS24400 Protein ID WP_004150355.1
Coordinates 5021162..5021404 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEY90_RS24380 5018145..5019047 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IEY90_RS24385 5019044..5019679 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IEY90_RS24390 5019676..5020605 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IEY90_RS24395 5020779..5021162 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IEY90_RS24400 5021162..5021404 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IEY90_RS24405 5021609..5022526 + 918 WP_004178029.1 alpha/beta hydrolase -
IEY90_RS24410 5022540..5023481 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IEY90_RS24415 5023526..5023963 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IEY90_RS24420 5023960..5024820 - 861 WP_004146232.1 virulence factor BrkB family protein -
IEY90_RS24425 5024814..5025413 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T173099 WP_002882817.1 NZ_CP061842:c5021162-5020779 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T173099 NZ_CP080099:1993723-1993825 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT173099 WP_004150355.1 NZ_CP061842:c5021404-5021162 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT173099 NZ_CP080099:c1993829-1993682 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References