Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 41881..42524 Replicon plasmid p1423_2
Accession NZ_CP061834
Organism Klebsiella pneumoniae strain C0674

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag IFX77_RS27405 Protein ID WP_000754566.1
Coordinates 41881..42297 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag IFX77_RS27410 Protein ID WP_001261276.1
Coordinates 42294..42524 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFX77_RS27380 38916..39251 + 336 Protein_37 Tn3 family transposase -
IFX77_RS27385 39469..39885 - 417 Protein_38 EstP -
IFX77_RS27390 39894..40340 - 447 Protein_39 restriction endonuclease -
IFX77_RS27395 40339..40539 - 201 Protein_40 IS110 family transposase -
IFX77_RS27400 40821..41677 - 857 Protein_41 IS3-like element ISEc15 family transposase -
IFX77_RS27405 41881..42297 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
IFX77_RS27410 42294..42524 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IFX77_RS27415 43098..43448 + 351 WP_000493378.1 hypothetical protein -
IFX77_RS27420 43499..44242 + 744 WP_000129823.1 hypothetical protein -
IFX77_RS27425 44239..45015 + 777 WP_000015958.1 site-specific integrase -
IFX77_RS27430 45073..45330 - 258 WP_000764642.1 hypothetical protein -
IFX77_RS27435 45459..45563 - 105 WP_032409716.1 hypothetical protein -
IFX77_RS27440 46093..46959 + 867 WP_004118283.1 replication initiation protein -
IFX77_RS27445 47136..47405 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / blaCTX-M-2 / blaOXA-1 / aac(6')-Ib-cr / tet(A) - 1..47819 47819


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T173077 WP_000754566.1 NZ_CP061834:c42297-41881 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T173077 NZ_CP080091:c4073235-4072951 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT173077 WP_001261276.1 NZ_CP061834:c42524-42294 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT173077 NZ_CP080091:c4073467-4073225 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References