Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5288761..5289386 Replicon chromosome
Accession NZ_CP061832
Organism Klebsiella pneumoniae strain C0674

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IFX77_RS25750 Protein ID WP_002882817.1
Coordinates 5288761..5289144 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IFX77_RS25755 Protein ID WP_004150355.1
Coordinates 5289144..5289386 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFX77_RS25735 5286127..5287029 + 903 WP_004901686.1 formate dehydrogenase subunit beta -
IFX77_RS25740 5287026..5287661 + 636 WP_004901683.1 formate dehydrogenase cytochrome b556 subunit -
IFX77_RS25745 5287658..5288587 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IFX77_RS25750 5288761..5289144 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IFX77_RS25755 5289144..5289386 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IFX77_RS25760 5289591..5290508 + 918 WP_004901674.1 alpha/beta hydrolase -
IFX77_RS25765 5290522..5291463 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IFX77_RS25770 5291508..5291945 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IFX77_RS25775 5291942..5292802 - 861 WP_002882807.1 virulence factor BrkB family protein -
IFX77_RS25780 5292796..5293395 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T173076 WP_002882817.1 NZ_CP061832:c5289144-5288761 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T173076 NZ_CP080091:c4032134-4031826 [Salmonella enterica]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT173076 WP_004150355.1 NZ_CP061832:c5289386-5289144 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT173076 NZ_CP080091:c4032376-4032137 [Salmonella enterica]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References