Detailed information of TA system TA173056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 4247..5355
• Replicon: plasmid pA6521_3
• Accession: NZ_CP061820
• Organism: Enterococcus faecium strain A6521

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: HI837_RS15550
• Protein ID: WP_000233000.1
• Coordinates: 4247..5116 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: HI837_RS15555
• Protein ID: WP_000205227.1
• Coordinates: 5131..5355 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI837_RS15525	392..1072	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
HI837_RS15530	1165..1959	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
HI837_RS15535	2052..2594	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
HI837_RS15540	2591..3499	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
HI837_RS15545	3532..4266	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
HI837_RS15550	4247..5116	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
HI837_RS15555	5131..5355	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
HI837_RS15560	5498..7060	-	1563	WP_000136908.1	recombinase family protein	-
HI837_RS15565	7062..7478	-	417	WP_000323438.1	recombinase	-
HI837_RS15570	7621..8301	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
HI837_RS15575	8499..9104	+	606	WP_000599739.1	cell filamentation protein	-
HI837_RS15580	9120..9692	+	573	WP_000170424.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T173056 WP_000233000.1 NZ_CP061820:c5116-4247 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T173056 NZ_CP080085:3994234-3994632 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT173056 WP_000205227.1 NZ_CP061820:c5355-5131 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT173056 NZ_CP080085:3993938-3994231 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1