Detailed information of TA system TA173055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 49079..49907
• Replicon: plasmid pA6521_p2
• Accession: NZ_CP061819
• Organism: Enterococcus faecium strain A6521

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: HI837_RS15235
• Protein ID: WP_080440613.1
• Coordinates: 49079..49633 (-)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: HI837_RS15240
• Protein ID: WP_000301765.1
• Coordinates: 49635..49907 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI837_RS15195	44194..45105	+	912	WP_000732308.1	VanY-A/VanY-F/VanY-M family D-Ala-D-Ala carboxypeptidase	-
HI837_RS15200	45258..45743	+	486	WP_000516404.1	glycopeptide resistance protein VanZ-A	-
HI837_RS15205	46030..46359	-	330	WP_000754864.1	metalloregulator ArsR/SmtB family transcription factor	-
HI837_RS15210	46664..46993	+	330	WP_000629052.1	hypothetical protein	-
HI837_RS15215	46983..47270	+	288	WP_000312841.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HI837_RS15220	47549..47764	-	216	Protein_51	recombinase family protein	-
HI837_RS15225	47809..48315	-	507	Protein_52	IS6 family transposase	-
HI837_RS15230	48345..49025	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
HI837_RS15235	49079..49633	-	555	WP_080440613.1	zeta toxin family protein	Toxin
HI837_RS15240	49635..49907	-	273	WP_000301765.1	antitoxin	Antitoxin
HI837_RS15245	50024..50230	+	207	Protein_56	IS6 family transposase	-
HI837_RS15250	50305..50985	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
HI837_RS15255	51141..51713	+	573	WP_182482952.1	hypothetical protein	-
HI837_RS15260	51870..52031	+	162	WP_154599257.1	hypothetical protein	-
HI837_RS15265	52833..54005	+	1173	WP_002323245.1	IS256-like element IS1542 family transposase	-
HI837_RS15270	54057..54419	-	363	Protein_61	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	VanHAX	-	1..99040	99040
	-	inside	IScluster/Tn	VanHAX	-	31371..54005	22634

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-179)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20526.23 Da
• Isoelectric Point: 5.7290

>T173055 WP_080440613.1 NZ_CP061819:c49633-49079 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHGSVAKF

Nucleotide

• Length: 555 bp

>T173055 NZ_CP080085:c3987403-3987125 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT173055 WP_000301765.1 NZ_CP061819:c49907-49635 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT173055 NZ_CP080085:c3987666-3987406 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3