Detailed information of TA system TA173054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 156661..157798
• Replicon: plasmid pA6521_1
• Accession: NZ_CP061818
• Organism: Enterococcus faecium strain A6521

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: HI837_RS14665
• Protein ID: WP_002401483.1
• Coordinates: 156661..157524 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: HI837_RS14670
• Protein ID: WP_000301765.1
• Coordinates: 157526..157798 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI837_RS14635	152568..154058	-	1491	WP_002380753.1	primase C-terminal domain-containing protein	-
HI837_RS14640	154453..154692	-	240	WP_002380754.1	helix-turn-helix domain-containing protein	-
HI837_RS14645	154936..155109	-	174	WP_171902975.1	rRNA adenine methyltransferase	-
HI837_RS14650	155054..155791	-	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
HI837_RS14655	155916..155981	-	66	Protein_160	23S rRNA methyltransferase attenuation leader peptide	-
HI837_RS14660	156034..156117	-	84	Protein_161	peptide-binding protein	-
HI837_RS14665	156661..157524	-	864	WP_002401483.1	zeta toxin family protein	Toxin
HI837_RS14670	157526..157798	-	273	WP_000301765.1	antitoxin	Antitoxin
HI837_RS14675	157815..158024	-	210	WP_000527317.1	peptide-binding protein	-
HI837_RS14680	158101..158997	-	897	WP_118215883.1	ParA family protein	-
HI837_RS14685	159100..159360	-	261	Protein_166	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
HI837_RS14690	159530..160267	-	738	WP_094899423.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
HI837_RS14695	160392..160487	-	96	WP_001809736.1	23S rRNA methyltransferase attenuation leader peptide	-
HI837_RS14700	160536..160775	-	240	WP_000635249.1	peptide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'') / erm(B)	-	1..213054	213054
	-	inside	IScluster/Tn	aac(6')-aph(2'') / erm(B)	-	143072..164145	21073

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-213)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32486.06 Da
• Isoelectric Point: 6.9965

>T173054 WP_002401483.1 NZ_CP061818:c157524-156661 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T173054 NZ_CP080085:c3614583-3613666 [Escherichia coli]
ATGGATAAAACCTCCCCCTACTACCGCCAGGTCGCTCTGTTGGTGCGAGCATTACCCTGGGTCGCTAACGAGTCCTGTTT
TGCGCTGAAAGGCGGAACGGCGATCAACCTATTCATCCATGATTTTCCCCGATTGTCCGTTGATATCGATCTGGCCTGGA
TACCGCTGGAAAGCAGAGAGGTCGCGCTGCCCAACGTGCGGGAGGCGCTCACCCGTATCGCCGCAGAACTGGAAGCGCAA
ACCGGGATCCGTGCAGTGCTGCAAACCCATAATCCCGATGAGATGCGCATCATTGTCACCACTGACGATGCGCAAATTAA
AATTGAAGTCTCGCCAGTTGCCCGGGGAACGCTATATCCTCCGCAAGAACGCGATATCGTCGGAGCCGTGGAGGAAGAAT
TTGGCTTTGCCGCGCTCCCGGTGGTCTCACTGCCCGATTTATACGGTGGTAAGCTATGTGCTGCGTTAGATCGCCAGCAC
CCCAGAGATTTTTACGACGTCAAAATGCTCCTTGATACTCAAGGAATTGATCGCTCGATATTCAATGGTTTTATCGCGTA
CTTGCTCAGCCATAACCGCCCATTATCCGAAGTGCTTAACCCTCGCTGGAAGGATATTACTGAACCCTTTCAAAATGAAT
TCAGCGGTATGACTTTTGAGGAAGTATCACTGGAAGAGCTAAAAGAGGTCCCGGAAAGAATGCTCAGCGCGTTGAAAACG
CATTTCACTCAACAGGATTTCGACTTCCTGCTGTCATTTAAACGCGGCGAGCCTGACTGGAATATAGCGCCAGAGCCCCG
CATTCAGAATTTGCCAGCCGTTCAATGGAAGCTGCGCAATATTGGACAGATGTCGGAGGAAAAGCGCGCGCAATCACTGG
AGAAACTAAAAAAGGTCATGACAGAATGGCTACGTTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT173054 WP_000301765.1 NZ_CP061818:c157798-157526 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT173054 NZ_CP080085:c3615349-3614576 [Escherichia coli]
ATGGCATCAAAATTAAACTGGCTGTTGCAAAACACCTCTCCGGGAAGCCTGGTGTTACAATCGTGGCTGACCCGGAACAA
CATATCACCTTCTCTGGCTTTTAAATACGCGCAAAATGGCTGGTTAATAAAACTGCGGGCTGGCGTTTATGCGCGCGCAG
GACGAGAACCAGACTGGAGTGATGCGCTATGGTGCCTACAAAACCAACTTGAAGCGCCGGTGCATCTGGCCGGACTGAGT
AGTCTCTCCTGGCAGGGCCGTTCCCATTACCTACAACTTAAGCAGAACCAATGCTGGCTTAGCGTTGAAAACAAAGCGAT
ATTGCCAAAATGGTTTAAAGAATTTCCCGGCGTTGAGTGGTTGATGGTATCGGCCCTGAAGCTCCCTGACCTGGATGAAA
AATACCGAACTGAGCTTGAGGTTAAAGGGAAAAGAATTACCGCCAGCACGCCAGAACTGGCTGCCTATGAGCTGTTAAGC
GCAGTCCCTCAGGAAATCTCATTTGAGCACGCTGCCGAGCTTTTTCAGGGACTAGTCAATCTCAACCCACGTAAAGTGGA
GAAGCTGCTTACACTCAGTCAGTCCGTACAAACCAAGCGCCTGTATCTGTTTTTTGCCAGTTTCTATGAACATGCATGGT
TCAAACGCATAGATACCAGCAAAATCAACCTGGGATCGGGCAACCGACAAATCGTTGTTAATGGCAAGCTGAACACGCAA
TACCAGATAACTGTGCCCGAAAATTTTACTAAGAAGGGAATAAGCCATGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3