Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 1019993..1020552 Replicon chromosome
Accession NZ_CP061816
Organism Brucella pinnipedialis strain 23a-1

Toxin (Protein)


Gene name brnT Uniprot ID D6LP74
Locus tag IEN84_RS10390 Protein ID WP_004688321.1
Coordinates 1019993..1020238 (+) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID D0RHG9
Locus tag IEN84_RS10395 Protein ID WP_006161014.1
Coordinates 1020238..1020552 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEN84_RS10360 1014996..1015436 - 441 Protein_949 hypothetical protein -
IEN84_RS10365 1015506..1016263 + 758 WP_080723468.1 IS5-like element IS711 family transposase -
IEN84_RS10370 1016275..1017486 - 1212 Protein_951 hypothetical protein -
IEN84_RS10375 1017621..1018394 - 774 WP_002967634.1 hypothetical protein -
IEN84_RS10385 1019222..1019860 - 639 Protein_954 tRNA-guanine transglycosylase -
IEN84_RS10390 1019993..1020238 + 246 WP_004688321.1 BrnT family toxin Toxin
IEN84_RS10395 1020238..1020552 + 315 WP_006161014.1 BrnA antitoxin family protein Antitoxin
IEN84_RS10400 1020900..1021070 + 171 WP_002964095.1 DUF1508 domain-containing protein -
IEN84_RS10405 1021418..1022128 - 711 WP_004683739.1 porin family protein -
IEN84_RS10410 1022362..1022949 - 588 WP_004683738.1 hypothetical protein -
IEN84_RS10415 1022972..1023247 - 276 WP_002964092.1 hypothetical protein -
IEN84_RS10420 1023244..1023474 - 231 WP_002964091.1 hypothetical protein -
IEN84_RS10425 1023471..1024189 - 719 Protein_962 hypothetical protein -
IEN84_RS10430 1024239..1024442 + 204 WP_002964088.1 hypothetical protein -
IEN84_RS10435 1024439..1024654 + 216 WP_006161017.1 hypothetical protein -
IEN84_RS10440 1024657..1024860 + 204 WP_002964087.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wbdA / wboA 1011883..1026632 14749


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9172.86 Da        Isoelectric Point: 10.3158

>T173051 WP_004688321.1 NZ_CP061816:1019993-1020238 [Brucella pinnipedialis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Download         Length: 246 bp

>T173051 NZ_CP080085:c2797283-2797176 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGTATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11668.45 Da        Isoelectric Point: 10.0945

>AT173051 WP_006161014.1 NZ_CP061816:1020238-1020552 [Brucella pinnipedialis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGWPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT173051 NZ_CP080085:2797341-2797395 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB D0RHG9

References