Detailed information of TA system TA173001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 1015915..1016523
• Replicon: chromosome
• Accession: NZ_CP061780
• Organism: Pseudomonas aeruginosa strain ZBX-P11

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A509JBR4
• Locus tag: IFJ77_RS04915
• Protein ID: WP_031638390.1
• Coordinates: 1015915..1016262 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: IFJ77_RS04920
• Protein ID: WP_003114155.1
• Coordinates: 1016272..1016523 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ77_RS04885 (IFJ77_04885)	1011334..1011546	+	213	WP_015503462.1	cysteine-rich CWC family protein	-
IFJ77_RS04890 (IFJ77_04890)	1011546..1012238	+	693	WP_015503463.1	16S rRNA pseudouridine(516) synthase	-
IFJ77_RS04895 (IFJ77_04895)	1012374..1013417	+	1044	WP_021264274.1	L,D-transpeptidase	-
IFJ77_RS04900 (IFJ77_04900)	1013497..1014234	+	738	WP_021264273.1	murein L,D-transpeptidase catalytic domain family protein	-
IFJ77_RS04905 (IFJ77_04905)	1014686..1015588	+	903	WP_003085447.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
IFJ77_RS04915 (IFJ77_04915)	1015915..1016262	-	348	WP_031638390.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IFJ77_RS04920 (IFJ77_04920)	1016272..1016523	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IFJ77_RS04925 (IFJ77_04925)	1016737..1017720	-	984	WP_034014422.1	tyrosine-type recombinase/integrase	-
IFJ77_RS04930 (IFJ77_04930)	1017720..1019012	-	1293	WP_003123045.1	hypothetical protein	-
IFJ77_RS04935 (IFJ77_04935)	1019271..1020533	-	1263	WP_021264270.1	zonular occludens toxin domain-containing protein	-
IFJ77_RS04940 (IFJ77_04940)	1020535..1020885	-	351	WP_003159569.1	DUF2523 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1013497..1024894	11397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13018.79 Da
• Isoelectric Point: 4.4212

>T173001 WP_031638390.1 NZ_CP061780:c1016262-1015915 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLVPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T173001 NZ_CP080075:1298365-1298763 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT173001 WP_003114155.1 NZ_CP061780:c1016523-1016272 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT173001 NZ_CP080075:1298138-1298365 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: A0A509JBR4

Antitoxin

• AlphaFold DB: A0A0B0C355