Detailed information of TA system TA172999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 6148834..6149339
• Replicon: chromosome
• Accession: NZ_CP061779
• Organism: Pseudomonas aeruginosa strain ZBX-P12

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: IFJ78_RS28645
• Protein ID: WP_003083773.1
• Coordinates: 6148834..6149115 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: IFJ78_RS28650
• Protein ID: WP_003083775.1
• Coordinates: 6149112..6149339 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ78_RS28620 (IFJ78_28625)	6144085..6145434	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
IFJ78_RS28625 (IFJ78_28630)	6145483..6146169	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
IFJ78_RS28630 (IFJ78_28635)	6146270..6147004	+	735	WP_003117208.1	GntR family transcriptional regulator	-
IFJ78_RS28635 (IFJ78_28640)	6147184..6147594	+	411	WP_003110659.1	aegerolysin family protein	-
IFJ78_RS28640 (IFJ78_28645)	6147626..6148534	-	909	WP_003083769.1	LysR family transcriptional regulator	-
IFJ78_RS28645 (IFJ78_28650)	6148834..6149115	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
IFJ78_RS28650 (IFJ78_28655)	6149112..6149339	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
IFJ78_RS28655 (IFJ78_28660)	6149514..6150134	-	621	WP_003101226.1	hypothetical protein	-
IFJ78_RS28660 (IFJ78_28665)	6150235..6150735	+	501	WP_003162763.1	LEA type 2 family protein	-
IFJ78_RS28665 (IFJ78_28670)	6150808..6151149	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
IFJ78_RS28670 (IFJ78_28675)	6151231..6152658	-	1428	WP_003083784.1	GABA permease	-
IFJ78_RS28675 (IFJ78_28680)	6152827..6154320	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T172999 WP_003083773.1 NZ_CP061779:c6149115-6148834 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T172999 NZ_CP080075:867080-867487 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT172999 WP_003083775.1 NZ_CP061779:c6149339-6149112 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT172999 NZ_CP080075:866833-867099 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9