Detailed information of TA system TA172997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 3810719..3811977
• Replicon: chromosome
• Accession: NZ_CP061779
• Organism: Pseudomonas aeruginosa strain ZBX-P12

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: IFJ78_RS17610
• Protein ID: WP_003163133.1
• Coordinates: 3810719..3811474 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: IFJ78_RS17615
• Protein ID: WP_003086329.1
• Coordinates: 3811609..3811977 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ78_RS17600 (IFJ78_17595)	3805891..3808434	+	2544	WP_003163132.1	acyl-homoserine-lactone acylase QuiP	-
IFJ78_RS17605 (IFJ78_17600)	3808589..3809950	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
IFJ78_RS17610 (IFJ78_17605)	3810719..3811474	-	756	WP_003163133.1	RES family NAD+ phosphorylase	Toxin
IFJ78_RS17615 (IFJ78_17610)	3811609..3811977	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
IFJ78_RS17620 (IFJ78_17615)	3812050..3813336	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
IFJ78_RS17625 (IFJ78_17620)	3813392..3814885	-	1494	WP_277915554.1	aldehyde dehydrogenase family protein	-
IFJ78_RS17630 (IFJ78_17625)	3815060..3815536	+	477	WP_003163135.1	hypothetical protein	-
IFJ78_RS17635 (IFJ78_17630)	3815539..3815814	-	276	WP_010792073.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28250.37 Da
• Isoelectric Point: 7.7294

>T172997 WP_003163133.1 NZ_CP061779:c3811474-3810719 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T172997 NZ_CP080075:296262-296537 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT172997 WP_003086329.1 NZ_CP061779:c3811977-3811609 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT172997 NZ_CP080075:296534-296893 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: V6AJU7