Detailed information of TA system TA172992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 654661..656356
• Replicon: chromosome
• Accession: NZ_CP061779
• Organism: Pseudomonas aeruginosa strain ZBX-P12

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: IFJ78_RS03005
• Protein ID: WP_048910032.1
• Coordinates: 654661..655530 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: IFJ78_RS03010
• Protein ID: WP_003112902.1
• Coordinates: 655523..656356 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ78_RS02980 (IFJ78_02980)	650669..651520	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
IFJ78_RS02985 (IFJ78_02985)	651561..652568	+	1008	WP_003162724.1	ABC transporter substrate-binding protein	-
IFJ78_RS02990 (IFJ78_02990)	652565..653341	+	777	WP_003112898.1	ABC transporter permease	-
IFJ78_RS02995 (IFJ78_02995)	653347..654108	+	762	WP_079274766.1	SDR family oxidoreductase	-
IFJ78_RS03000 (IFJ78_03000)	654122..654652	+	531	WP_003162721.1	cupin domain-containing protein	-
IFJ78_RS03005 (IFJ78_03005)	654661..655530	+	870	WP_048910032.1	alpha/beta hydrolase	Toxin
IFJ78_RS03010 (IFJ78_03010)	655523..656356	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
IFJ78_RS03015 (IFJ78_03015)	656343..657140	+	798	WP_003112903.1	SDR family oxidoreductase	-
IFJ78_RS03020 (IFJ78_03020)	657133..658815	+	1683	WP_003112904.1	thiamine pyrophosphate-binding protein	-
IFJ78_RS03025 (IFJ78_03025)	658826..659629	+	804	WP_003112905.1	aspartate dehydrogenase	-
IFJ78_RS03030 (IFJ78_03030)	659641..661125	+	1485	WP_003163202.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30336.60 Da
• Isoelectric Point: 6.5894

>T172992 WP_048910032.1 NZ_CP061779:654661-655530 [Pseudomonas aeruginosa]
MNMAQTHIPAPSLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERLGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSPIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T172992 NZ_CP080070:3435597-3435815 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT172992 WP_003112902.1 NZ_CP061779:655523-656356 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT172992 NZ_CP080070:3435197-3435571 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: Q9HYA1