Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 6688772..6689380 Replicon chromosome
Accession NZ_CP061778
Organism Pseudomonas aeruginosa strain ZBX-P13

Toxin (Protein)


Gene name PfiT Uniprot ID A0A444LUU5
Locus tag IFJ79_RS30955 Protein ID WP_019486378.1
Coordinates 6688772..6689119 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag IFJ79_RS30960 Protein ID WP_003114155.1
Coordinates 6689129..6689380 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFJ79_RS30925 (IFJ79_30930) 6684191..6684403 + 213 WP_003085460.1 cysteine-rich CWC family protein -
IFJ79_RS30930 (IFJ79_30935) 6684403..6685095 + 693 WP_003085458.1 16S rRNA pseudouridine(516) synthase -
IFJ79_RS30935 (IFJ79_30940) 6685231..6686274 + 1044 WP_003134392.1 L,D-transpeptidase -
IFJ79_RS30940 (IFJ79_30945) 6686354..6687091 + 738 WP_003085453.1 murein L,D-transpeptidase catalytic domain family protein -
IFJ79_RS30945 (IFJ79_30950) 6687543..6688445 + 903 WP_003085447.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
IFJ79_RS30955 (IFJ79_30960) 6688772..6689119 - 348 WP_019486378.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IFJ79_RS30960 (IFJ79_30965) 6689129..6689380 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IFJ79_RS30965 (IFJ79_30970) 6689594..6690577 - 984 WP_033990852.1 tyrosine-type recombinase/integrase -
IFJ79_RS30970 (IFJ79_30975) 6690577..6691869 - 1293 WP_033990854.1 hypothetical protein -
IFJ79_RS30975 (IFJ79_30980) 6692099..6693373 - 1275 WP_033990855.1 zonular occludens toxin family protein -
IFJ79_RS30980 (IFJ79_30985) 6693377..6693733 - 357 WP_003114150.1 DUF2523 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 6688772..6711864 23092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12956.72 Da        Isoelectric Point: 4.4212

>T172991 WP_019486378.1 NZ_CP061778:c6689119-6688772 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T172991 NZ_CP080070:2681625-2681732 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT172991 WP_003114155.1 NZ_CP061778:c6689380-6689129 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT172991 NZ_CP080070:c2681578-2681512 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A444LUU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References