Detailed information of TA system TA172989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 5408297..5409517
• Replicon: chromosome
• Accession: NZ_CP061778
• Organism: Pseudomonas aeruginosa strain ZBX-P13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A3S3XIW1
• Locus tag: IFJ79_RS24905
• Protein ID: WP_033991668.1
• Coordinates: 5408939..5409517 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IFJ79_RS24900
• Protein ID: WP_003088112.1
• Coordinates: 5408297..5408851 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ79_RS24875 (IFJ79_24880)	5403865..5404416	+	552	WP_003132721.1	XRE family transcriptional regulator	-
IFJ79_RS24880 (IFJ79_24885)	5404413..5404811	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
IFJ79_RS24885 (IFJ79_24890)	5404824..5405147	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
IFJ79_RS24890 (IFJ79_24895)	5405478..5405939	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
IFJ79_RS24895 (IFJ79_24900)	5405944..5408139	+	2196	WP_003139882.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
IFJ79_RS24900 (IFJ79_24905)	5408297..5408851	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
IFJ79_RS24905 (IFJ79_24910)	5408939..5409517	+	579	WP_033991668.1	HD domain-containing protein	Toxin
IFJ79_RS24910 (IFJ79_24915)	5409627..5410814	-	1188	WP_003132729.1	HlyD family efflux transporter periplasmic adaptor subunit	-
IFJ79_RS24915 (IFJ79_24920)	5410804..5412975	-	2172	WP_023106617.1	type I secretion system permease/ATPase	-
IFJ79_RS24920 (IFJ79_24925)	5412965..5414242	-	1278	WP_003088104.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21947.77 Da
• Isoelectric Point: 5.3655

>T172989 WP_033991668.1 NZ_CP061778:5408939-5409517 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLFQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T172989 NZ_CP080070:2321147-2321434 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT172989 WP_003088112.1 NZ_CP061778:5408297-5408851 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT172989 NZ_CP080070:2320908-2321147 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A3S3XIW1