Detailed information of TA system TA172983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 6991301..6991806
• Replicon: chromosome
• Accession: NZ_CP061777
• Organism: Pseudomonas aeruginosa strain ZBX-P23

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: IFJ80_RS32775
• Protein ID: WP_003083773.1
• Coordinates: 6991301..6991582 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: IFJ80_RS32780
• Protein ID: WP_003083775.1
• Coordinates: 6991579..6991806 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ80_RS32750 (IFJ80_32755)	6986552..6987901	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
IFJ80_RS32755 (IFJ80_32760)	6987950..6988636	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
IFJ80_RS32760 (IFJ80_32765)	6988737..6989471	+	735	WP_003117208.1	GntR family transcriptional regulator	-
IFJ80_RS32765 (IFJ80_32770)	6989651..6990061	+	411	WP_003110659.1	aegerolysin family protein	-
IFJ80_RS32770 (IFJ80_32775)	6990093..6991001	-	909	WP_003083769.1	LysR family transcriptional regulator	-
IFJ80_RS32775 (IFJ80_32780)	6991301..6991582	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
IFJ80_RS32780 (IFJ80_32785)	6991579..6991806	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
IFJ80_RS32785 (IFJ80_32790)	6991981..6992601	-	621	WP_003101226.1	hypothetical protein	-
IFJ80_RS32790 (IFJ80_32795)	6992702..6993202	+	501	WP_003162763.1	LEA type 2 family protein	-
IFJ80_RS32795 (IFJ80_32800)	6993275..6993616	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
IFJ80_RS32800 (IFJ80_32805)	6993698..6995125	-	1428	WP_003083784.1	GABA permease	-
IFJ80_RS32805 (IFJ80_32810)	6995294..6996787	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T172983 WP_003083773.1 NZ_CP061777:c6991582-6991301 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T172983 NZ_CP080070:766902-767198 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT172983 WP_003083775.1 NZ_CP061777:c6991806-6991579 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT172983 NZ_CP080070:767200-767595 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9