Detailed information of TA system TA172977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1668562..1670070
• Replicon: chromosome
• Accession: NZ_CP061777
• Organism: Pseudomonas aeruginosa strain ZBX-P23

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: IFJ80_RS07940
• Protein ID: WP_079276297.1
• Coordinates: 1668562..1669149 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A5B3ABU1
• Locus tag: IFJ80_RS07945
• Protein ID: WP_003119683.1
• Coordinates: 1669216..1670070 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ80_RS07935 (IFJ80_07935)	1664817..1668296	-	3480	WP_003117605.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
IFJ80_RS07940 (IFJ80_07940)	1668562..1669149	+	588	WP_079276297.1	GNAT family protein	Toxin
IFJ80_RS07945 (IFJ80_07945)	1669216..1670070	+	855	WP_003119683.1	AraC family transcriptional regulator	Antitoxin
IFJ80_RS07950 (IFJ80_07950)	1670167..1672332	+	2166	WP_003115567.1	TonB-dependent receptor family protein	-
IFJ80_RS07955 (IFJ80_07955)	1672507..1674381	+	1875	WP_003117607.1	MFS transporter	-
IFJ80_RS07960 (IFJ80_07960)	1674442..1674651	-	210	WP_003115568.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21892.22 Da
• Isoelectric Point: 9.8675

>T172977 WP_079276297.1 NZ_CP061777:1668562-1669149 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQQGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T172977 NZ_CP080066:c4257344-4257171 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31875.19 Da
• Isoelectric Point: 7.5295

>AT172977 WP_003119683.1 NZ_CP061777:1669216-1670070 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Nucleotide

• Length: 855 bp

>AT172977 NZ_CP080066:c4257668-4257372 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A5B3ABU1