Detailed information of TA system TA172976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1410197..1411892
• Replicon: chromosome
• Accession: NZ_CP061777
• Organism: Pseudomonas aeruginosa strain ZBX-P23

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: IFJ80_RS06715
• Protein ID: WP_048910032.1
• Coordinates: 1410197..1411066 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: IFJ80_RS06720
• Protein ID: WP_003112902.1
• Coordinates: 1411059..1411892 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ80_RS06690 (IFJ80_06690)	1406205..1407056	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
IFJ80_RS06695 (IFJ80_06695)	1407097..1408104	+	1008	WP_003162724.1	ABC transporter substrate-binding protein	-
IFJ80_RS06700 (IFJ80_06700)	1408101..1408877	+	777	WP_003112898.1	ABC transporter permease	-
IFJ80_RS06705 (IFJ80_06705)	1408883..1409644	+	762	WP_079274766.1	SDR family oxidoreductase	-
IFJ80_RS06710 (IFJ80_06710)	1409658..1410188	+	531	WP_003162721.1	cupin domain-containing protein	-
IFJ80_RS06715 (IFJ80_06715)	1410197..1411066	+	870	WP_048910032.1	alpha/beta hydrolase	Toxin
IFJ80_RS06720 (IFJ80_06720)	1411059..1411892	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
IFJ80_RS06725 (IFJ80_06725)	1411879..1412676	+	798	WP_003112903.1	SDR family oxidoreductase	-
IFJ80_RS06730 (IFJ80_06730)	1412669..1414351	+	1683	WP_003112904.1	thiamine pyrophosphate-binding protein	-
IFJ80_RS06735 (IFJ80_06735)	1414362..1415165	+	804	WP_003112905.1	aspartate dehydrogenase	-
IFJ80_RS06740 (IFJ80_06740)	1415177..1416661	+	1485	WP_003163202.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30336.60 Da
• Isoelectric Point: 6.5894

>T172976 WP_048910032.1 NZ_CP061777:1410197-1411066 [Pseudomonas aeruginosa]
MNMAQTHIPAPSLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERLGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSPIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T172976 NZ_CP080066:c4153717-4153367 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT172976 WP_003112902.1 NZ_CP061777:1411059-1411892 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT172976 NZ_CP080066:c4153962-4153711 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCTTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: Q9HYA1