Detailed information of TA system TA172974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 84144..84787
• Replicon: plasmid unnamed1
• Accession: NZ_CP061765
• Organism: Escherichia coli O19:H7 strain 730V1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: ID627_RS25810
• Protein ID: WP_016236302.1
• Coordinates: 84371..84787 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: ID627_RS25805
• Protein ID: WP_001261282.1
• Coordinates: 84144..84374 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ID627_RS25770	79598..81133	-	1536	WP_023063425.1	IS66 family transposase	-
ID627_RS25775	81183..81530	-	348	WP_000609174.1	IS66 family insertion sequence element accessory protein TnpB	-
ID627_RS25780	81527..81910	-	384	WP_001201739.1	IS66 family insertion sequence hypothetical protein	-
ID627_RS25785	81968..82441	-	474	Protein_91	tyrosine-type recombinase/integrase	-
ID627_RS25790	82438..83181	-	744	WP_004098973.1	hypothetical protein	-
ID627_RS25795	83232..83582	-	351	WP_000493378.1	hypothetical protein	-
ID627_RS25800	83726..84187	-	462	WP_170989135.1	hypothetical protein	-
ID627_RS25805	84144..84374	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ID627_RS25810	84371..84787	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
ID627_RS25815	84861..86423	+	1563	WP_004206609.1	AAA family ATPase	-
ID627_RS25820	86408..87430	+	1023	WP_000361404.1	helicase UvrD	-
ID627_RS25830	88359..88580	+	222	Protein_100	IS5/IS1182 family transposase	-
ID627_RS25835	88729..89046	-	318	Protein_101	hypothetical protein	-
ID627_RS25840	89043..89528	-	486	Protein_102	plasmid transfer protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..98015	98015

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T172974 WP_016236302.1 NZ_CP061765:84371-84787 [Escherichia coli O19:H7]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T172974 NZ_CP080066:c3674247-3673882 [Escherichia coli]
ATGAAAACTCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTTCTTAC
ACGGCTACTGAAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATCAAACCGTTATACAGGAACATATCG
ATGCCGGTATCACTCTGGCTAATGCTGTTAACTTTCTTGTAGAAAAGCATGAGTTAGTACGTATCGATCGCACAGGATTT
ACCTCACAGGAACAAGCGCCATATTTGACTGTGACTGATATCCTTCATGCCAGGAGAGCATGCGGATTAATGAACAGTTG
TTCGTATCGGGAAGTAAGTAATATTGTTCTTAGCCGATCGCGGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT172974 WP_001261282.1 NZ_CP061765:84144-84374 [Escherichia coli O19:H7]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT172974 NZ_CP080066:c3674585-3674268 [Escherichia coli]
ATGAGCAACATCACATGGGGCCTGCAGCGGGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAATGCCTGAAGTTGGATGTCGCATTCCCGC
AATTTATCAGTCAGATGGAATCAATGCTGACTACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCATCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCTTTGGTAGTTGCGGCTACGTATATATCGCCATTTATCCCATTCAACGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3