Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4009404..4010191 Replicon chromosome
Accession NZ_CP061764
Organism Escherichia coli O19:H7 strain 730V1

Toxin (Protein)


Gene name yeeV Uniprot ID A0A377E3D6
Locus tag ID627_RS19970 Protein ID WP_001194695.1
Coordinates 4009814..4010191 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2H9G3E7
Locus tag ID627_RS19965 Protein ID WP_000066236.1
Coordinates 4009404..4009763 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ID627_RS19920 4004644..4005096 + 453 WP_001020418.1 hypothetical protein -
ID627_RS19925 4005093..4005545 + 453 WP_000734138.1 hypothetical protein -
ID627_RS19930 4005608..4006150 + 543 WP_001104018.1 DUF4339 domain-containing protein -
ID627_RS19935 4006209..4006661 + 453 WP_001061894.1 hypothetical protein -
ID627_RS19940 4006738..4006971 + 234 WP_001114682.1 DUF905 domain-containing protein -
ID627_RS19945 4007091..4007909 + 819 WP_001234406.1 DUF945 domain-containing protein -
ID627_RS19950 4008177..4008647 + 471 WP_000131762.1 antirestriction protein -
ID627_RS19955 4008659..4009138 + 480 WP_000437750.1 DNA repair protein RadC -
ID627_RS19960 4009159..4009380 + 222 WP_000691981.1 DUF987 domain-containing protein -
ID627_RS19965 4009404..4009763 + 360 WP_000066236.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ID627_RS19970 4009814..4010191 + 378 WP_001194695.1 TA system toxin CbtA family protein Toxin
ID627_RS19975 4010188..4010679 + 492 WP_000777682.1 hypothetical protein -
ID627_RS19980 4010711..4010914 + 204 WP_000413747.1 DUF957 domain-containing protein -
ID627_RS19985 4010995..4011839 + 845 Protein_3939 DUF4942 domain-containing protein -
ID627_RS19995 4012181..4012912 - 732 WP_001300756.1 DNA polymerase III subunit epsilon -
ID627_RS20000 4012977..4013444 + 468 WP_000917883.1 ribonuclease HI -
ID627_RS20005 4013441..4014163 - 723 WP_001295200.1 class I SAM-dependent methyltransferase -
ID627_RS20010 4014197..4014952 + 756 WP_001052721.1 hydroxyacylglutathione hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(15-114)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14180.97 Da        Isoelectric Point: 7.4209

>T172971 WP_001194695.1 NZ_CP061764:4009814-4010191 [Escherichia coli O19:H7]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKATGLMTRNDYRTVTDITTGKYRGGHR

Download         Length: 378 bp

>T172971 NZ_CP080066:3423555-3423773 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13127.02 Da        Isoelectric Point: 7.0261

>AT172971 WP_000066236.1 NZ_CP061764:4009404-4009763 [Escherichia coli O19:H7]
MSNKIPTVNHDITEPWWGLKRSITPCFGARLVQSGNCLHYLADRASITGQFSDGDLRHLDQAFPLLLKQLELMLTSGELT
PRHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Download         Length: 360 bp

>AT172971 NZ_CP080066:3423155-3423529 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377E3D6

Antitoxin

Source ID Structure
AlphaFold DB A0A2H9G3E7

References